Simple one-step procedure, takes 1-2 hours. Detection method: Absorbance at 400 or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.

This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab39401)

Scientific Data Images for Caspase-3 Activity Assay Kit (Colorimetric)

N/A: Caspase-3 Activity Assay Kit (Colorimetric) [NBP2-54838] -

N/A: Caspase-3 Activity Assay Kit (Colorimetric) [NBP2-54838] - Induction of Caspase-3 activity by Anti-Fas antibody in Jurkat-T cells using Caspase-3 Activity Assay Kit (Colorimetric).

Kit Contents for Caspase-3 Activity Assay Kit (Colorimetric)

2X Reaction Buffer
Cell Lysis Buffer
DEVD-pNA (4mM)
Dilution Buffer
DTT (1 M)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Caspase-3

CPP32 encodes a member of the cysteine-aspartic acid protease (caspase) family that plays a key role in apoptosis. Caspase 3 (also known as CPP32, Apopain, Yama, and PARP cleavage protease) is the most extensively studied apoptotic protein among caspase family members (1-2). Caspase 3 is synthesized as an inactive proenzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). Alternative splicing of CPP32 results in two transcript variants which encode the same protein. The processed form of Caspase 3 consists of large (theoretical molecular weight 17kD) and small (theoretical molecular weight 12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28/Ser29 and Asp175/Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Variations in levels of Caspase 3 have been reported in cells of short-lived nature and those with a longer life cycle. Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease (3).

References

1.Mu, N., Lei, Y., Wang, Y., Wang, Y., Duan, Q., Ma, G., . . . Su, L. (2019). Inhibition of SIRT1/2 upregulates HSPA5 acetylation and induces pro-survival autophagy via ATF4-DDIT4-mTORC1 axis in human lung cancer cells. Apoptosis, 24(9-10), 798-811. doi:10.1007/s10495-019-01559-3

2.Sun, C. M., Enkhjargal, B., Reis, C., Zhou, K. R., Xie, Z. Y., Wu, L. Y., . . . Zhang, J. H. (2019). Osteopontin attenuates early brain injury through regulating autophagy-apoptosis interaction after subarachnoid hemorrhage in rats. CNS Neurosci Ther, 25(10), 1162-1172. doi:10.1111/cns.13199

3.Louneva, N., Cohen, J. W., Han, L. Y., Talbot, K., Wilson, R. S., Bennett, D. A., . . . Arnold, S. E. (2008). Caspase-3 is enriched in postsynaptic densities and increased in Alzheimer's disease. Am J Pathol, 173(5), 1488-1495. doi:10.2353/ajpath.2008.080434

Alternate Names

Apopain, CASP3, Caspase3, CPP32, LICE-1, YAMA

Gene Symbol

CASP3

Additional Caspase-3 Products

Product Documents for Caspase-3 Activity Assay Kit (Colorimetric)

Product Datasheets

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Protocols & Manuals

Download Manual - Caspase-3 Activity Assay Kit (Colorimetric

COA

SDS

Download SDS

Product Specific Notices for Caspase-3 Activity Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Caspase-3 Activity Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54838

Key Product Details

Sample Type & Volume Required Per Well

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Product Specifications

Kit Type

Reactivity

Description