Could you advise on the best way to prepare a cell lysate for use with your beta-glucosidase assay kit (KA1611)?

Name: Glucosylceramidase/GBA Assay Kit (Colorimetric)
Brand: Novus Biologicals
SKU: KA1611

This particular product is made by a Taiwanese company called Abnova; we distribute their products in addition to selling our own. Here is a link to their protocol for KA1611. We suggest to prepare lysate in 50 mM phosphate (pH 7.0) buffer or in any other suitable enzyme buffer. However, the following chemicals are known to affect the enzyme activity and should be avoided: SH-containing reagents (e.g. dithiothreitol, 2-mercaptoethanol, glutathione), Ca2+, Cu2+, Fe3+/Fe2+, Hg2+, Mg2+, Ni2+, Zn2+, SDS, EDTA and Tris.

Glucosylceramidase/GBA Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA1611

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Product Specifications
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Biological (20 uL)

Sensitivity

2 U/L

Assay Range

2 to 250 U/L

View all Glucosylceramidase/GBA Activity Assays »

Product Specifications

Assay Type

Colorimetric

Kit Type

Assay Kit (Colorimetric)

Reactivity

Non-species specific

Description

beta-Glucosidase Assay Kit is a colorimetric kinetic determination of beta-Glucosidase activity.

Scientific Data Images for Glucosylceramidase/GBA Assay Kit (Colorimetric)

Enzyme Activity: Glucosylceramidase/GBA Assay Kit (Colorimetric) [KA1611]

Enzyme Activity: Glucosylceramidase/GBA Colorimetric Assay Kit [KA1611] - Kinetics of beta-glucosidase reaction in 96-well plate assay.

Kit Contents for Glucosylceramidase/GBA Assay Kit (Colorimetric)

Assay Buffer: (pH 7.0)
Calibrator: (equivalent to 250 U/L)
beta-NPG Substrate

Preparation and Storage

Formulation

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Glucosylceramidase/GBA

beta-GLUCOSIDASE is a glucosidase enzyme which acts upon beta 1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). beta-Glucosidases are required by organisms (some fungi, bacteria, termites) for consumption of cellulose. Lysozyme is also a beta-glucosidase and is present in tears to prevent bacterial infection of the eye. In humans, lower activity of a beta-glucosidase isoform (lysosomal gluco-cerebrosidase) has been related to Gaucher's disease and Parkinson's disease. Simple, direct and automation-ready procedures for measuring beta-glucosidase activity are becoming popular in Research and Drug Discovery. Beta-Glucosidase Assay Kit is designed to measure beta-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl- beta-D-glucopyranoside that is hydrolyzed specifically by beta-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity

Alternate Names

Alglucerase, GBA, GBA1, Imiglucerase

Gene Symbol

GBA1

Additional Glucosylceramidase/GBA Products

Product Documents for Glucosylceramidase/GBA Assay Kit (Colorimetric)

Product Datasheets

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Protocols & Manuals

Download Glucosylceramidase/GBA Assay Kit

COA

Product Specific Notices for Glucosylceramidase/GBA Assay Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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