Glutamate Dehydrogenase Activity Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24494

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, urine and animal tissue

Sensitivity

0.54 U /L

Assay Range

0.54 - 25.0 U/L

View all Glutamate Dehydrogenase 1/GLUD1 Activity Assays »

Product Specifications

Kit Type

Activity Assay Kit (Colorimetric)

Reactivity

Non-species specific

Description

Detection instrument: Microplate reader (440 - 460 nm, optimum wavelength: 450 nm)
Assay time: 35 min

Precision

Intra-Assay Precision (Precision within an assay) %CV < 3

Inter-Assay Precision (Precision between assays) %CV < 4

Recovery for Glutamate Dehydrogenase Activity Assay Kit (Colorimetric)

Recovery

Average recovery rate: 103%

Scientific Data Images for Glutamate Dehydrogenase Activity Assay Kit (Colorimetric)

Glutamate Dehydrogenase Activity Assay Kit (Colorimetric)

Glutamate Dehydrogenase Activity Assay Kit (Colorimetric) [NBP3-24494] -

Glutamate Dehydrogenase Activity Assay Kit (Colorimetric) [NBP3-24494] - Standard Reference Curve
Glutamate Dehydrogenase Activity Assay Kit (Colorimetric)

Glutamate Dehydrogenase Activity Assay Kit (Colorimetric) [NBP3-24494] -

Glutamate Dehydrogenase Activity Assay Kit (Colorimetric) [NBP3-24494] - Assay Example

Kit Contents for Glutamate Dehydrogenase Activity Assay Kit (Colorimetric)

  • Microplate
  • Plate Sealer
  • Reagent 1: Extracting Solution
  • Reagent 2: Buffer Solution
  • Reagent 3: Substrate A
  • Reagent 4: Substrate B
  • Reagent 5: Chromogenic Agent
  • Reagent 6: Standard

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Glutamate Dehydrogenase 1/GLUD1

One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1; EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.

Alternate Names

GDH, GDH1

Gene Symbol

GLUD1

Additional Glutamate Dehydrogenase 1/GLUD1 Products

Product Documents for Glutamate Dehydrogenase Activity Assay Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Glutamate Dehydrogenase Activity Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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