Hydroxyproline Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59747

Novus Biologicals, part of Bio-Techne
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Product Specifications

Kit Type

Assay Kit (Colorimetric)

Reactivity

Mammal

Description

This kit uses an acidic developer solution to accurately measure hydroxyproline in tissue and protein/peptidase hydrolysates without the use of toxic perchlorates. Featuring a simple and quick protocol, this hydroxyproline assay yields a brightly-colored chromophore with an absorbance peak at 560 nm. The assay can detect a minimum of 0.1 ug hydroxyproline per well in a 96-well format and generates colorimetric results that are virtually identical to the classical perchloric acid-based method.

This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab222941)

Scientific Data Images for Hydroxyproline Assay Kit (Colorimetric)

Hydroxyproline Colorimetric Assay Kit [NBP2-59747]
Hydroxyproline Colorimetric Assay Kit [NBP2-59747]

Kit Contents for Hydroxyproline Assay Kit (Colorimetric)

  • Chloramine T Concentrate
  • Developer Solution
  • DMAB Concentrate (in DMSO)
  • Hydroxyproline Standard (1 mg/ml)
  • Microplate Sealing Film
  • Oxidation Buffer

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 °C.

Background: Hydroxyproline

Hydroxyproline ((2S,4R)-4-hydroxyproline, Hyp) is a nonproteinogenic amino acid formed by the enzyme prolyl-4-hydroxylase, which catalizes the post-translational modification of polypeptide proline residues following protein synthesis. In animals, hydroxyproline is found almost entirely in collagen, which contains approximately 12-14% hydroxyproline by mass (the fibrilous protein elastin also contains small quantities of hydroxyproline). Hydroxyproline in tissue hydrolysates is thus a direct measure of the amount of collagen present. Collagen is the most abundant protein in mammals and many diseases are believed to affect collagen turnover, including tumor invasion and metastasis, rheumatoid arthritis, cardiopulmonary fibrosis and muscular dystrophy. The classical assay for determination of Hyp is based upon oxidation of Hyp to a pyrrole intermediate, followed by reaction with Ehrlich's reagent dissolved in concentrated perchloric acid (HClO4). Perchloric acid is a hazardous material that is both toxic and highly reactive, requiring special handling and waste-disposal protocols.

Additional Hydroxyproline Products

Product Documents for Hydroxyproline Assay Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Hydroxyproline Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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