PPAR gamma/NR1C3 Transcription Factor Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA1358

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Nuclear Extracts (10 uL/well. All samples should be assayed in duplicate at minimum.)

View all PPAR gamma/NR1C3 Activity Assays »

Product Specifications

Assay Type

Colorimetric

Kit Type

Transcription Factor Assay Kit (Colorimetric)

Description

PPAR gamma/NR1C3 Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting PPAR gamma/NR1C3 transcription factor DNA binding activity in nuclear extracts.

Scientific Data Images for PPAR gamma/NR1C3 Transcription Factor Assay Kit (Colorimetric)

PPAR gamma/NR1C3 Assay Kit (Colorimetric) [KA1358] - Standard Curve Example

Kit Contents for PPAR gamma/NR1C3 Transcription Factor Assay Kit (Colorimetric)

  • 96-Well Cover Sheet
  • Polysorbate 20
  • Transcription Factor Antibody Binding Buffer (10x)
  • Transcription Factor Binding Assay Buffer (4x)
  • Transcription Factor Developing Solution
  • Transcription Factor Goat Anti-Rabbit HRP Conjugate
  • Transcription Factor PPAR 96-Well Strip Plate
  • Transcription Factor PPAR Competitor dsDNA
  • Transcription Factor PPAR(gamma) Positive Control
  • Transcription Factor PPAR(gamma) Primary Antibody
  • Transcription Factor Reagent A
  • Transcription Factor Stop Solution
  • Wash Buffer Concentrate (400x)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PPAR gamma/NR1C3

Peroxisome proliferator-activated receptors (PPARs) are ligand-activated nuclear receptors. PPAR gamma is the most widely studied PPAR and exists in two protein isoforms (Gamma 1 and Gamma 2) due to use of an alternative promoter and alternative splicing. PPAR gamma is primarily expressed in adipose tissue and to a lesser extent in the colon, immune system, and the retina.

Long Name

Peroxisome Proliferator-activated Receptor gamma

Alternate Names

NR1C3, PPARG

Gene Symbol

PPARG

Additional PPAR gamma/NR1C3 Products

Product Documents for PPAR gamma/NR1C3 Transcription Factor Assay Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PPAR gamma/NR1C3 Transcription Factor Assay Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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