RelA/NFkB p65 Transcription Factor Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA1341

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Nuclear Extracts (10 uL/well. All samples should be assayed in duplicate at minimum.)

View all RelA/NFkB p65 Activity Assays »

Product Specifications

Assay Type

Colorimetric

Kit Type

Transcription Factor Assay Kit (Colorimetric)

Reactivity

Human, Mouse

Specificity

This NF-kB (p65) Transcription Factor Assay Kit detects human, mouse, and rat NF-kB (p65). It will not cross-react with NF-kB (p50).

Description

NF-kB (p65) Transcription Factor Assay Kit is a non-radioactive method for detecting specific transcription factor DNA binding activity.

Scientific Data Images for RelA/NFkB p65 Transcription Factor Assay Kit (Colorimetric)

RelA/NFkB p65 Assay Kit (Colorimetric) [KA1341] - Assay of cell lysates isolated from stimulated (20 ng/mL TNF-a for 30 minutes) and nonstimulated HeLa cells demonstrating NF-kB (p65) activity.

Kit Contents for RelA/NFkB p65 Transcription Factor Assay Kit (Colorimetric)

  • 96-Well Cover Sheet
  • Polysorbate 20
  • Transcription Factor Antibody Binding Buffer (10X)
  • Transcription Factor Binding Assay Buffer (4X)
  • Transcription Factor Developing Solution
  • Transcription Factor Goat Anti-Rabbit HRP Conjugate
  • Transcription Factor NF-kB (human p65) Positive Control
  • Transcription Factor NF-kB (p65) Primary Antibody
  • Transcription Factor NF-kB 96-Well Strip Plate
  • Transcription Factor NF-kB Competitor dsDNA
  • Transcription Factor Reagent A
  • Transcription Factor Stop Solution
  • Wash Buffer Concentrate (400X)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: RelA/NFkB p65

In the canonical pathway of NF-kB activation, p65/p50 heterodimers are normally sequestered in the cytoplasm by binding to IkBa. Upon stimulus, such as TNFR1 activation, IkBa is phosphorylated, ubiquitinated and degraded, allowing the translocation of p65/p50 to the nucleus. There, the transcription factor binds to specific DNA sequences, called kB site, and stimulates transcription of target genes. Since NF-kB is a powerful activator of proinflammatory transcriptional programs, its activity is tightly regulated. IkBa is a transcriptional target of p65/p50, resulting in a negative feedback loop and 'turning off' NF-kB activation. The importance of Rel/NF-kB transcription factors in human inflammation and certain diseases makes them attractive targets for potential therapeutcs

Long Name

v-rel Reticuloendotheliosis Viral Oncogene Homolog A

Alternate Names

NFkB p65, NFKB3, p65RelA

Gene Symbol

RELA

Additional RelA/NFkB p65 Products

Product Documents for RelA/NFkB p65 Transcription Factor Assay Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RelA/NFkB p65 Transcription Factor Assay Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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