Sirtuin 1/SIRT1 Assay Kit (Fluorometric)

Novus Biologicals, part of Bio-Techne | Catalog # KA1366

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Compounds expected to inhibit SIRT1 (5 uL/well. All samples should be assayed in duplicate at minimum.)

View all Sirtuin 1/SIRT1 Activity Assays »

Product Specifications

Assay Type

Fluorometric

Kit Type

Assay Kit (Fluorometric)

Reactivity

Mouse

Description

Sirtuin 1/SIRT1 Direct Fluorescent Screening Assay Kit provides a fluorescence-based method for screening SIRT1 inhibitors or activators.

Scientific Data Images for Sirtuin 1/SIRT1 Assay Kit (Fluorometric)

Sirtuin 1/SIRT1 Assay Kit (Fluorometric) [KA1366] - Inhibition of SIRT1 by Sirtinol (IC50= 38 uM).

Kit Contents for Sirtuin 1/SIRT1 Assay Kit (Fluorometric)

  • 96-Well Cover Sheet
  • Half Volume 96-Well Plate (white)
  • SIRT1 (human recombinant)
  • SIRT1 Direct Assay Buffer (10X)
  • SIRT1 Direct Developer
  • SIRT1 Direct Fluorophore
  • SIRT1 Direct NAD+
  • SIRT1 Direct Nicotinamide
  • SIRT1 Direct Peptide

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: Sirtuin 1/SIRT1

There are seven human sirtuins which have been designated SIRT1-SIRT7. SIRT1, which is located in the nucleus, is the human sirtuin with the greatest homology to yeast Silent information regulator 2 (Sir2) and has been shown to regulate the activity of the p53 tumor suppressor and inhibit apoptosis. These results have significant implications regarding an important role of SIRT1 in modulating the sensitivity of cells in p53-dependent apoptotic response and the possible effect in cancer therapy. Since the growth suppressive function of p53 is strongly enhanced by DNA damaging reagents, it is expected that inhibitors of SIRT1 may be effective anticancer drugs.

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2-like protein 1, SIR2alpha, SIR2L1, SIRT1

Gene Symbol

SIRT1

Additional Sirtuin 1/SIRT1 Products

Product Documents for Sirtuin 1/SIRT1 Assay Kit (Fluorometric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Sirtuin 1/SIRT1 Assay Kit (Fluorometric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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