11 beta-HSD1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-69644

Novus Biologicals, part of Bio-Techne
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NBP1-69644
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all 11 beta-HSD1 Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to 11 Beta HSD1(hydroxysteroid (11-beta) dehydrogenase 1) The peptide sequence was selected from the N terminal of 11 Beta HSD1. Peptide sequence QKVVSHCLELGAASAHYIAGTMEDMTFAEQFVAQAGKLMGGLDMLILNHI. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for 11 beta-HSD1 Antibody

Western Blot: 11 beta-HSD1 Antibody [NBP1-69644]

Western Blot: 11 beta-HSD1 Antibody [NBP1-69644]

Western Blot: 11 beta-HSD1 Antibody [NBP1-69644] - 11 Beta HSD1 This Anti-HSD11B1 antibody was used in Western Blot of Fetal Liver tissue lysate at a concentration of 1ug/ml.
Immunohistochemistry-Paraffin: 11 beta-HSD1 Antibody [NBP1-69644]

Immunohistochemistry-Paraffin: 11 beta-HSD1 Antibody [NBP1-69644]

Immunohistochemistry-Paraffin: 11 beta-HSD1 Antibody [NBP1-69644] - Human liver at 5.0 ug/ml
Western Blot: 11 beta-HSD1 Antibody [NBP1-69644]

Western Blot: 11 beta-HSD1 Antibody [NBP1-69644]

Western Blot: 11 beta-HSD1 Antibody [NBP1-69644] - Antibody Titration: 2 ug/ml Positive control: Transient overexpression lysate of HSD11B1 and Non-overexpressed vector control lysate.

Applications for 11 beta-HSD1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 11 beta-HSD1

11 Beta HSD1 is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, 11 Beta HSD1 can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children.The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Two transcript variants encoding the same protein have been found for this gene.

Long Name

11 beta Hydroxysteroid Dehydrogenase 1

Alternate Names

11 betaHSD1, HSD11B1

Gene Symbol

HSD11B1

UniProt

P28845

Additional 11 beta-HSD1 Products

Product Documents for 11 beta-HSD1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for 11 beta-HSD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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