11 beta-HSD1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27469

Novus Biologicals, part of Bio-Techne
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NBP2-27469
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all 11 beta-HSD1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TARSEEGLQKVVSR corresponding to internal region according to NP_032314.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for 11 beta-HSD1 Antibody

Western Blot: 11 beta-HSD1 Antibody [NBP2-27469]

Western Blot: 11 beta-HSD1 Antibody [NBP2-27469]

Western Blot: 11 beta-HSD1 Antibody [NBP2-27469] - (0.3 ug/ml) Analysis of Mouse Lung lysate (60 ug protein in RIPA buffer). First lane shows wildtype and second lane shows knockout background. Primary incubation was overnight. Detected by fluorescence. Data obtained from Prof. K. Chapman and Zhenguang Zhang, Centre for Cardiovascular Sciences, Queen's Medical Research Institute, Edinburgh, UK
Western Blot: 11 beta-HSD1 Antibody [NBP2-27469]

Western Blot: 11 beta-HSD1 Antibody [NBP2-27469]

Western Blot: 11 beta-HSD1 Antibody [NBP2-27469] - (0.3 ug/ml) Analysis of Mouse Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for 11 beta-HSD1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 32 kDa band observed in mouse lung lysates (calculated MW of 32.4 kDa band according to NP_032314.2). An additional band of unknown identity was also consistently observed at 45 kDa. This band was successfully blocked by incubation with the immunizing peptide. However, it remained visible in a KO background.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 11 beta-HSD1

HSD11B1 is encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Two transcript variants encoding the same protein have been found for this gene.

Long Name

11 beta Hydroxysteroid Dehydrogenase 1

Alternate Names

11 betaHSD1, HSD11B1

Entrez Gene IDs

15483 (Mouse); 25116 (Rat)

Gene Symbol

HSD11B1

UniProt

NP_032314.2 (Human)

Additional 11 beta-HSD1 Products

Product Documents for 11 beta-HSD1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for 11 beta-HSD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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