12-Lipoxygenase Antibody
Novus Biologicals, part of Bio-Techne | Catalog # H00000239-B01P
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Mouse IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ALOX12 (NP_000688, 1 a.a. - 663 a.a.) full-length human protein. MGRYRIRVATGAWLFSGSYNRVQLWLVGTRGEAELELQLRPARGEEEEFDHDVAEDLGLLQFVRLRKHHWLVDDAWFCDRITVQGPGACAEVAFPCYRWVQGEDILSLPEGTARLPGDNALDMFQKHREKELKDRQQIYCWATWKEGLPLTIAADRKDDLPPNMRFHEEKRLDFEWTLKAGALEMALKRVYTLLSSWNCLEDFDQIFWGQKSALAEKVRQCWQDDELFSYQFLNGANPMLLRRSTSLPSRLVLPSGMEELRAQLEKELQNGSLFEADFILLDGIPANVIRGEKQYLAAPLVMLKMEPNGKLQPMVIQIQPPNPSSPTPTLFLPSDPPLAWLLAKSWVRNSDFQLHEIQYHLLNTHLVAEVIAVATMRCLPGLHPIFKFLIPHIRYTMEINTRARTQLISDGGIFDKAVSTGGGGHVQLLRRAAAQLTYCSLCPPDDLADRGLLGLPGALYAHDALRLWEIIARYVEGIVHLFYQRDDIVKGDPELQAWCREITEVGLCQAQDRGFPVSFQSQSQLCHFLTMCVFTCTAQHAAINQGQLDWYAWVPNAPCTMRMPPPTTKEDVTMATVMGSLPDVRQACLQMAISWHLSRRQPDMVPLGHHKEKYFSGPKPKAVLNQFRTDLEKLEKEITARNEQLDWPYEYLKPSCIENSVTI
Specificity
ALOX12 - arachidonate 12-lipoxygenase,
Clonality
Polyclonal
Host
Mouse
Isotype
IgG
Description
Quality control test: Antibody reactive against mammalian transfected lysate.
Scientific Data Images for 12-Lipoxygenase Antibody
![Western Blot: 12-Lipoxygenase Antibody [H00000239-B01P]](https://resources.bio-techne.com/images/products/12-Lipoxygenase-Antibody-Western-Blot-H00000239-B01P-img0004.jpg "Western Blot: 12-Lipoxygenase Antibody [H00000239-B01P]")
Western Blot: 12-Lipoxygenase Antibody [H00000239-B01P]
Western Blot: 12-Lipoxygenase Antibody [H00000239-B01P] - Analysis of ALOX12 expression in transfected 293T cell line by ALOX12 polyclonal antibody. Lane 1: ALOX12 transfected lysate(72.93 KDa). Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: 12-Lipoxygenase Antibody [H00000239-B01P]](https://resources.bio-techne.com/images/products/12-Lipoxygenase-Antibody-Immunocytochemistry-Immunofluorescence-H00000239-B01P-img0003.jpg "Immunocytochemistry/ Immunofluorescence: 12-Lipoxygenase Antibody [H00000239-B01P]")
Immunocytochemistry/ Immunofluorescence: 12-Lipoxygenase Antibody [H00000239-B01P]
Immunocytochemistry/Immunofluorescence: 12-Lipoxygenase Antibody [H00000239-B01P] - Analysis of purified antibody to ALOX12 on HeLa cell. (antibody concentration 10 ug/ml)Applications for 12-Lipoxygenase Antibody
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for IF.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: 12-Lipoxygenase
Long Name
Polyunsaturated fatty acid lipoxygenase ALOX12
Alternate Names
12LO, 12S-lipoxygenase, 12S-LOX, ALOX12, EC 1.13.11.-, EC 1.13.11.31, EC 1.13.11.33, EC 3.3.2.-, LOG12
Entrez Gene IDs
239 (Human)
Gene Symbol
ALOX12
UniProt
Additional 12-Lipoxygenase Products
Product Documents for 12-Lipoxygenase Antibody
Product Specific Notices for 12-Lipoxygenase Antibody
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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