14-3-3 beta Antibody (03)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06516
![Immunocytochemistry/ Immunofluorescence: 14-3-3 beta Antibody (03) [NBP3-06516]](https://resources.bio-techne.com/images/products/14-3-3-beta-Antibody-03-Immunocytochemistry-Immunofluorescence-NBP3-06516-img0001.jpg "Immunocytochemistry/ Immunofluorescence: 14-3-3 beta Antibody (03) [NBP3-06516]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 03
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 beta (Accession#: NP_003395.1; Met1-Asn246).
Reactivity Notes
Has cross-reactivity in ELISA with:
Cynomolgus YWHAB
No cross-reactivity in ELISA with:
E.coli cell lysate (BL21 cell line)
Cynomolgus YWHAB
No cross-reactivity in ELISA with:
E.coli cell lysate (BL21 cell line)
Specificity
Has cross-reactivity in ELISA with: Cynomolgus YWHABNo cross-reactivity in ELISA with: E.coli cell lysate (BL21 cell line)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for 14-3-3 beta Antibody (03)
Immunocytochemistry/ Immunofluorescence: 14-3-3 beta Antibody (03) [NBP3-06516]
Immunocytochemistry/Immunofluorescence: 14-3-3 beta Antibody (03) [NBP3-06516] - Staining of Human YWHAB in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human YWHAB monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.![Flow Cytometry: 14-3-3 beta Antibody (03) [NBP3-06516]](https://resources.bio-techne.com/images/products/14-3-3-beta-Antibody-03-Flow-Cytometry-NBP3-06516-img0002.jpg "Flow Cytometry: 14-3-3 beta Antibody (03) [NBP3-06516]")
Flow Cytometry: 14-3-3 beta Antibody (03) [NBP3-06516]
Flow Cytometry: 14-3-3 beta Antibody (03) [NBP3-06516] - Analysis of Human YWHAB expression on Molt-4 cells. The cells were treated, stained with purified anti-Human YWHAB, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.Applications for 14-3-3 beta Antibody (03)
Application
Recommended Usage
ELISA
1:1000-1:2000
Flow Cytometry
1:25-1:100
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: 14-3-3 beta
Alternate Names
1433 beta, GW128, HS1, YWHAB
Gene Symbol
YWHAB
Additional 14-3-3 beta Products
Product Documents for 14-3-3 beta Antibody (03)
Product Specific Notices for 14-3-3 beta Antibody (03)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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