14-3-3 epsilon Antibody (CPTC-YWHAE-1) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79944
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # CPTC-YWHAE-1
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human full-length 14-3-3 epsilon protein (Uniprot: P62268)
Localization
Cytoplasm, Melanosome
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
29.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79810).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for 14-3-3 epsilon Antibody (CPTC-YWHAE-1) - Azide and BSA Free
Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1)Azide and BSA Free [NBP2-79944]
Western Blot: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) - Azide and BSA Free [NBP2-79944] - Western Blot Analysis of HEK293, PC-3 and A431 cell lysates using 14-3-3E Mouse Monoclonal Antibody (CPTC-YWHAE-1).Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) - Azide and BSA Free [NBP2-79944]
Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) - Azide and BSA Free [NBP2-79944] - Formalin-fixed, paraffin-embedded human Testis stained with 14-3-3E Mouse Monoclonal Antibody (CPTC-YWHAE-1).Protein Array: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) - Azide and BSA Free [NBP2-79944]
Protein Array: 14-3-3 epsilon Antibody (CPTC-YWHAE-1) - Azide and BSA Free [NBP2-79944] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.Applications for 14-3-3 epsilon Antibody (CPTC-YWHAE-1) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1-2 ug/mL
Immunohistochemistry-Paraffin
1-2 ug/mL
Western Blot
1-2 ug/mL
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: 14-3-3 epsilon
Alternate Names
1433 epsilon, YWHAE
Gene Symbol
YWHAE
Additional 14-3-3 epsilon Products
Product Documents for 14-3-3 epsilon Antibody (CPTC-YWHAE-1) - Azide and BSA Free
Product Specific Notices for 14-3-3 epsilon Antibody (CPTC-YWHAE-1) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...