14-3-3 epsilon Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33554
![Western Blot: 14-3-3 epsilon Antibody [NBP1-33554]](https://resources.bio-techne.com/images/products/14-3-3-epsilon-Antibody-Western-Blot-NBP1-33554-img0004.jpg "Western Blot: 14-3-3 epsilon Antibody [NBP1-33554]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Primate (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for 14-3-3 epsilon Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human 14-3-3 epsilon. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Chimpanzee (100%).
Localization
Cytoplasm, Melanosome
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for 14-3-3 epsilon Antibody
![Western Blot: 14-3-3 epsilon Antibody [NBP1-33554]](https://resources.bio-techne.com/images/products/14-3-3-epsilon-Antibody-Western-Blot-NBP1-33554-img0005.jpg "Western Blot: 14-3-3 epsilon Antibody [NBP1-33554]")
Western Blot: 14-3-3 epsilon Antibody [NBP1-33554]
Western Blot: 14-3-3 epsilon Antibody [NBP1-33554] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:1000.![Immunocytochemistry/ Immunofluorescence: 14-3-3 epsilon Antibody [NBP1-33554]](https://resources.bio-techne.com/images/products/14-3-3-epsilon-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33554-img0002.jpg "Immunocytochemistry/ Immunofluorescence: 14-3-3 epsilon Antibody [NBP1-33554]")
Immunocytochemistry/ Immunofluorescence: 14-3-3 epsilon Antibody [NBP1-33554]
Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP1-33554] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.![Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-33554]](https://resources.bio-techne.com/images/products/14-3-3-epsilon-Antibody-Immunohistochemistry-Paraffin-NBP1-33554-img0003.jpg "Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-33554]")
Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-33554]
Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-33554] - Paraffin-embedded MDA-MB231 xenograft, using antibody at 1:500 dilution.Applications for 14-3-3 epsilon Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: 14-3-3 epsilon
Alternate Names
1433 epsilon, YWHAE
Gene Symbol
YWHAE
Additional 14-3-3 epsilon Products
Product Documents for 14-3-3 epsilon Antibody
Product Specific Notices for 14-3-3 epsilon Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.