14-3-3 gamma Antibody (6A10)

Novus Biologicals, part of Bio-Techne | Catalog # H00007532-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 6A10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all 14-3-3 gamma Antibodies »

Product Specifications

Immunogen

YWHAG (NP_036611, 70 a.a. ~ 169 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TSADGNEKKIEMVRAYREKIEKELEAVCQDVLSLLDNYLIKNCSETQYESKVFYLKMKGDYYRYLAEVATGEKRATVVESSEKAYSEAHEISKEHMQPTH

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

YWHAG - tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for 14-3-3 gamma Antibody (6A10)

Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02]

Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02]

Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in NIH/3T3.
Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02]

Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02]

Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in PC-12.
Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02]

Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02]

Western Blot: 14-3-3 gamma Antibody (6A10) [H00007532-M02] - YWHAG monoclonal antibody (M02), clone 6A10. Analysis of YWHAG expression in IMR-32.
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Applications for 14-3-3 gamma Antibody (6A10)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: 14-3-3 gamma

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

Alternate Names

1433 gamma, YWHAG

Entrez Gene IDs

7532 (Human)

Gene Symbol

YWHAG

OMIM

605356 (Human)

UniProt

NP_036611 (Human)

Additional 14-3-3 gamma Products

Product Documents for 14-3-3 gamma Antibody (6A10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for 14-3-3 gamma Antibody (6A10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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