14-3-3 sigma/Stratifin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2465

Novus Biologicals, part of Bio-Techne
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NB100-2465
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all 14-3-3 sigma Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DLHTLSEDSYKDST corresponding to internal region according to NP_006133.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for 14-3-3 sigma/Stratifin Antibody

Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465]

Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465]

Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - Staining of Mouse (A) and Rat (B) Skin lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NB100-2465]

Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NB100-2465]

Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - Staining of paraffin embedded human skin. Antibody at 2 ug/mL. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. Image shows strong nuclear staining in selected cells of the basal layer.
Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465]

Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465]

Western Blot: 14-3-3 sigma/Stratifin Antibody [NB100-2465] - (0.1ug/ml) staining of human skin lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Applications for 14-3-3 sigma/Stratifin Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 4 ug/mL

Immunohistochemistry-Paraffin

2 - 4 ug/mL

Peptide ELISA

Detection limit 1:16000

Western Blot

0.03 - 0.1 ug/mL
Application Notes
WB: Approx. 28 kDa band observed in human skin lysates (calculated MW of 27.8 kDa band according to NP_006133.1). IHC-P: Human skin shows strong nuclear staining in selected cells of the basal layer.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 14-3-3 sigma

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.

Alternate Names

1433 sigma, Epithelial cell marker protein 1, SFN, YWHAS

Entrez Gene IDs

2810 (Human); 55948 (Mouse); 313017 (Rat)

Gene Symbol

SFN

UniProt

NP_006133.1 (Human)

Additional 14-3-3 sigma Products

Product Documents for 14-3-3 sigma/Stratifin Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for 14-3-3 sigma/Stratifin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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