14-3-3 sigma/Stratifin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27072

Novus Biologicals, part of Bio-Techne
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NBP2-27072
NBP2-27072SS
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Key Product Details

Species Reactivity

Validated:

Human, Amphibian, Bovine, Canine, Equine, Opossum, Primate

Predicted:

Chimpanzee (100%), Monkey (100%), Mouse (100%), Orangutan (100%), Porcine (100%), Rabbit (100%), Rat (100%), Rhesus Macaque (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all 14-3-3 sigma Antibodies »

Product Specifications

Immunogen

A portion of amino acids 120-170 of human 14-3-3 sigma was used as the immunogen for this antibody.

Reactivity Notes

The amino acid sequence used as immunogen for the antibody is 100% homologous in White-cheeked Gibbon, and 89% in Opossum.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 14-3-3 sigma/Stratifin Antibody

Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072]

Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072]

Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - Analysis of 14-3-3 sigma (169 amino acid isoform) using 14-3-3 sigma antibody. Human brain tissue in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain tissue probed with 14-3-3 sigma antibody at 3 ug/ml.
Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NBP2-27072]

Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NBP2-27072]

Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - Formalin-fixed, paraffin-embedded human tonsil (squamous epithelium) probed with 14-3-3 sigma antibody at 10 ug/ml.
Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072]

Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072]

Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - analysis of 14-3-3 sigma (248 amino acid isoform) using 14-3-3 sigma polyclonal antibody. Human A431 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) HeLa cell lysate probed with 14-3-3 sigma antibody at 3 ug/ml.

Applications for 14-3-3 sigma/Stratifin Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5-10ug/ml

Western Blot

3-5ug/ml~

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 14-3-3 sigma

14-3-3 sigma (stratifin, SFN, epithelial cell marker protein 1) is a member of a highly conserved family of 14-3-3 proteins that are present in all eukaryotic organisms. There are 7 known mammalian 14-3-3 isoforms (sigma epsilon, beta, zeta, gamma, theta, tau and they play important roles in many biological activities by binding to and altering the subcellular localization and/or stability of key molecules in various signaling cascades. 14-3-3 sigma was originally characterized as a human mammary epithelium marker and later rediscovered as an important molecule for cell cycle checkpoint regulation. Recently it has been reported that 14-3-3 sigma may serve as a prognosis marker predicting survival of pancreatic cancer patient treatment. 14-3-3 sigma is the only 14-3-3 isoform induced by the tumor suppressor protein p53, in response to irradiation and other DNA-damaging agents. 14-3-3 sigma is a p53-regulated inhibitor of G2/M progression and acts as a tumor suppressor gene that is inactivated by methylation of its 5' CpG islands in epithelial tumor cells. Two isoforms of human 14-3-3 sigma are produced by alternative splicing.

Alternate Names

1433 sigma, Epithelial cell marker protein 1, SFN, YWHAS

Gene Symbol

SFN

UniProt

P31947

Additional 14-3-3 sigma Products

Product Documents for 14-3-3 sigma/Stratifin Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for 14-3-3 sigma/Stratifin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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