14-3-3 sigma/Stratifin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99631

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all 14-3-3 sigma Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human 14-3-3 sigma/Stratifin (Accession#: NP_006133.1; Met1-Ser248).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for 14-3-3 sigma/Stratifin Antibody

Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-99631]

Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-99631]

Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-99631] - Anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lane C: PC12 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 33 kDa (We are unsure as to the identity of these extra bands).
Immunocytochemistry/ Immunofluorescence: 14-3-3 sigma/Stratifin Antibody [NBP2-99631]

Immunocytochemistry/ Immunofluorescence: 14-3-3 sigma/Stratifin Antibody [NBP2-99631]

Immunocytochemistry/Immunofluorescence: 14-3-3 sigma/Stratifin Antibody [NBP2-99631] - Immunofluorescence staining of 14-3-3 sigma/Stratifin in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human 14-3-3 sigma/Stratifin polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Immunoprecipitation: 14-3-3 sigma/Stratifin Antibody [NBP2-99631]

Immunoprecipitation: 14-3-3 sigma/Stratifin Antibody [NBP2-99631]

Immunoprecipitation: 14-3-3 sigma/Stratifin Antibody [NBP2-99631] - 14-3-3 sigma/Stratifin was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate. 2 ul anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-14-3-3 sigma/Stratifin rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 32 kDa

Applications for 14-3-3 sigma/Stratifin Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 14-3-3 sigma

14-3-3 sigma was identified as an epithelial cell marker. It is part of the 14-3-3 family of proteins in which seven isoforms have been identified: beta, zeta, gamma, eta, epsilon, tau, and sigma. 14-3-3 proteins function as adaptors that bind with a number of partners to mediate various signaling pathways. 14-3-3 sigma is also called stratifin or HME1 and appears to function as a tumor suppressor whose expression can be downregulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis. Alternate names for 14-3-3 sigma include epithelial marker protein 1, SFN and YWHAS.

Alternate Names

1433 sigma, Epithelial cell marker protein 1, SFN, YWHAS

Gene Symbol

SFN

Additional 14-3-3 sigma Products

Product Documents for 14-3-3 sigma/Stratifin Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for 14-3-3 sigma/Stratifin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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