14-3-3 zeta Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24593
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
A portion of amino acids 200-245 of human 14-3-3 zeta was used as the immunogen for the antibody.
Reactivity Notes
The immunogen shows 100% sequence identity in human, cattle, rat, mouse, chimpanzee, rhesus monkey, opossum, pig, chicken and dog, and 94% in frog.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for 14-3-3 zeta Antibody
Western Blot: 14-3-3 zeta Antibody [NBP2-24593]
Western Blot: 14-3-3 zeta Antibody [NBP2-24593] - analysis of 14-3-3 zeta in human brain lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain lysate and 4) rat brain lysate in the absence of immunizing peptide using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP2-24593]
Immunohistochemistry-Paraffin: 14-3-3 zeta Antibody [NBP2-24593] - Formalin-fixed, paraffin-embedded human spleen stained with 14-3-3 zeta antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.Applications for 14-3-3 zeta Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
10 ug/ml
Western Blot
1-3 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: 14-3-3 zeta
Alternate Names
1433 zeta, KCIP-1, YWHAZ
Gene Symbol
YWHAZ
Additional 14-3-3 zeta Products
Product Documents for 14-3-3 zeta Antibody
Product Specific Notices for 14-3-3 zeta Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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