17 beta-HSD1/HSD17B1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84901

Novus Biologicals, part of Bio-Techne
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NBP1-84901
NBP1-84901-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all 17 beta-HSD1/HSD17B1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FGVHLSLIECGPVHTAFMEKVLGSPEEVLDRTDIHTFHRFYQYLAHSKQVFREAAQNPEEVAEVFLTALRAPKPTL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 17 beta-HSD1/HSD17B1 Antibody

Immunocytochemistry/ Immunofluorescence: 17 beta-HSD1/HSD17B1 Antibody [NBP1-84901]

Immunocytochemistry/ Immunofluorescence: 17 beta-HSD1/HSD17B1 Antibody [NBP1-84901]

Immunocytochemistry/Immunofluorescence: 17 beta-HSD1/HSD17B1 Antibody [NBP1-84901] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: 17 beta-HSD1/HSD17B1 Antibody [NBP1-84901]

Immunohistochemistry-Paraffin: 17 beta-HSD1/HSD17B1 Antibody [NBP1-84901]

Immunohistochemistry-Paraffin: 17 beta-HSD1/HSD17B1 Antibody [NBP1-84901] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: 17 beta-HSD1/HSD17B1 Antibody [NBP1-84901]

Immunohistochemistry-Paraffin: 17 beta-HSD1/HSD17B1 Antibody [NBP1-84901]

Immunohistochemistry-Paraffin: 17 beta-HSD1/HSD17B1 Antibody [NBP1-84901] - Staining of human placenta shows strong nuclear and cytoplasmic positivity in Syncytiotrophoblasts.
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Applications for 17 beta-HSD1/HSD17B1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 17 beta-HSD1/HSD17B1

The principal human estrogen, 17 beta-estradiol, is a potent stimulator of certain endocrine-dependent forms of breast cancer. Because human estrogenic 17 beta-hydroxysteroid dehydrogenase (HSD17B1) catalyzes the last step in the biosynthesis of 17 beta-estradiol from the less potent estrogen, estrone, it is an attractive target for the design of inhibitors of estrogen production and tumor growth (1). It is concluded that the steroid-binding site of human placental HSD17B1 contains a histidine residue which proximates the upper A-ring region of the steroid as it undergoes the reversible binding step (2). Human estrogenic HSD17B1 is an NADP(H)-preferring enzyme. It possesses 11- and 4-fold higher specificity toward NADP(H) over NAD(H) for oxidation and reduction, respectively, as demonstrated by kinetic studies (3). Defects in the conversion of androstenedione to testosterone in the fetal testes by the enzyme HSD17B1 give rise to genetic males with female external genitalia (4)

Long Name

17 beta Hydroxysteroid Dehydrogenase 1

Alternate Names

17 betaHSD1, E2DH, EDH17B2, EDHB17, HSD17B1, SDR28C1

Gene Symbol

HSD17B1

Additional 17 beta-HSD1/HSD17B1 Products

Product Documents for 17 beta-HSD1/HSD17B1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for 17 beta-HSD1/HSD17B1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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