2B4/CD244/SLAMF4 Antibody (2B4.69) [Alexa Fluor® 350]
Novus Biologicals, part of Bio-Techne | Catalog # NB100-65889AF350
![2B4/CD244/SLAMF4 Antibody (2B4.69) [Alexa Fluor® 350]](https://resources.bio-techne.com/images/products/nb100-65889af350_mouse-monoclonal-2b4-cd244-slamf4-antibody-2b4-69-alexa-fluor-350-2982023930202.jpg "2B4/CD244/SLAMF4 Antibody (2B4.69) [Alexa Fluor® 350] [NB100-65889AF350] -")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunoprecipitation
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 2B4.69
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for 2B4/CD244/SLAMF4 Antibody (2B4.69) [Alexa Fluor® 350]
Immunogen
CD244 Fusion protein
Localization
Cell Membrane; single-pass type I membrane protein (potential).
Specificity
NB100-65889 is specific for the CD244 cell surface antigen, also known as natural killer receptor 2B4 (NKG2B4). The glycoprotein CD244 is a member of the SLAM family of receptors which is expressed on natural killer (NK) cells, gamma delta T cells, CD14+ monocytes, and a subset of CD8+ T cells. CD244 was originally identified as an activating receptor on NK cells, although recent studies suggest that the receptor may have a wide range of activities including NK cell activation, costimulation, or even inhibition, depending on the cell type and stage of differentiation. The ligand of 2B4 is CD48, a GPI-anchored membrane protein also belonging to the SLAM family.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for 2B4/CD244/SLAMF4 Antibody (2B4.69) [Alexa Fluor® 350]
2B4/CD244/SLAMF4 Antibody (2B4.69) [Alexa Fluor® 350] [NB100-65889AF350] -
2B4/CD244/SLAMF4 Antibody (2B4.69) [Alexa Fluor® 350] [NB100-65889AF350] - Vial of Alexa Fluor 350 conjugated antibody. Alexa Fluor 350 is optimally excited at 346 nm by the UV laser (350 or 355 nm) and has an emission maximum of 442 nm.Applications for 2B4/CD244/SLAMF4 Antibody (2B4.69) [Alexa Fluor® 350]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: 2B4/CD244/SLAMF4
The 2B4 receptor has both activating and inhibitory signaling properties (1,4). 2B4's ligand is CD48, which is expressed on hematopoietic cells (4, 5). When bound to CD48, subsequent phosphorylation of the ITSMs occurs, followed by recruitment of SH-2-containing adaptor protein (SAP) and Fyn, leading to phosphorylation of PLCgamma and Vav1, and resulting in NK cell activation (4). In the absence of SAP, Src homology region 2-containing protein tyrosine phosphatases (SHP) get recruited and inhibits NK cell activation (4). In the context of infection such as Epstein-Barr virus (EBV), when lacking SAP, 2B4 has been shown to have an inhibitory function via impaired T cell cytotoxicity against EBV-infected cells (6). While 2B4 has a role in eliciting CD8+ T cell response, overexpression of 2B4 can lead to T cell exhaustion in states of chronic infection (6). Studies have suggested that the antibody blockade of the 2B4-CD48 interaction might enhance T cell and NK cell function, however given both the activating and inhibitory properties of the 2B4 receptor, more research needs to be done to prevent unwanted effects (6).
References
1. Buller CW, Mathew PA, Mathew SO. Roles of NK Cell Receptors 2B4 (CD244), CS1 (CD319), and LLT1 (CLEC2D) in Cancer. Cancers (Basel). 2020 Jul 1;12(7):1755. https://doi.org/10.3390/cancers12071755
2. Catakovic K, Klieser E, Neureiter D, Geisberger R. T cell exhaustion: from pathophysiological basics to tumor immunotherapy. Cell Commun Signal. 2017;15(1):1. https://doi.org/10.1186/s12964-016-0160-z
3. Uniprot (Q9BZW8)
4. Chen Y, Lu D, Churov A, Fu R. Research Progress on NK Cell Receptors and Their Signaling Pathways. Mediators Inflamm. 2020 Jul 24;2020:6437057. https://doi.org/10.1155/2020/6437057.
5. Pahima H, Puzzovio PG, Levi-Schaffer F. 2B4 and CD48: A powerful couple of the immune system. Clin Immunol. 2019 Jul;204:64-68. https://doi.org/10.1016/j.clim.2018.10.014.
6. Waggoner SN, Kumar V. Evolving role of 2B4/CD244 in T and NK cell responses during virus infection. Front Immunol. 2012;3:377. https://doi.org/10.3389/fimmu.2012.00377
Alternate Names
CD244, NAIL, NKR2B4, Nmrk, SLAMF4
Gene Symbol
CD244
Additional 2B4/CD244/SLAMF4 Products
Product Documents for 2B4/CD244/SLAMF4 Antibody (2B4.69) [Alexa Fluor® 350]
Product Specific Notices for 2B4/CD244/SLAMF4 Antibody (2B4.69) [Alexa Fluor® 350]
Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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