Key Product Details

Species Reactivity

Human, All Species

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # P3UI

Format

Azide and BSA Free

Concentration

2.6 mg/ml

View all 3-OHKYN Antibodies »

Product Specifications

Immunogen

3-OHKYN modified KLH

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 3-OHKYN Antibody (P3UI) - Azide and BSA Free

Western Blot: 3-OHKYN Antibody (P3UI)Azide and BSA Free [NBP2-80548]

Western Blot: 3-OHKYN Antibody (P3UI)Azide and BSA Free [NBP2-80548]

Western Blot: 3-OHKYN Antibody (P3UI) - Azide and BSA Free [NBP2-80548] - Detection of 3'OHKYN in modified BSA using NB 100-597. 10 second ECL exposure. Lane 1: BSA (-) Lane 2: BSA (+). Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: 3-OHKYN Antibody (P3UI) - Azide and BSA Free [NBP2-80548]

Immunohistochemistry-Paraffin: 3-OHKYN Antibody (P3UI) - Azide and BSA Free [NBP2-80548]

Immunohistochemistry-Paraffin: 3-OHKYN Antibody (P3UI) - Azide and BSA Free [NBP2-80548] - 3-OHKYN was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human 3-OHKYN (P3UI) Monoclonal Antibody (Catalog # NB100-597) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCy

Applications for 3-OHKYN Antibody (P3UI) - Azide and BSA Free

Application
Recommended Usage

ELISA

reported in scientific literature

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunohistochemistry-Paraffin

reported in scientific literature

Western Blot

2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

2.6 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: 3-OHKYN

3-OHKYN (3-Hydroxykynurenine) is tryptophan's toxic metabolite which is capable of inducing oxidative damage as well as cell death consequently contributing to different neurological and psychiatric disorders. Tryptophan is either transaminated to kynurenate (an endogenous glutamate receptor antagonist), or hydroxylated to 3-OHKYN which is degraded to excitotoxic N-methyl-D-aspartate (NMDA) agonist, quinolinate. Under physiological conditions, kynurenine pathway is activated to form metabolic precursor NAD+; however, under inflammatory, oxidative or neurotoxic attack, there is an increased activation of indoleamine dioxygenase (IDO), kynurenine monooxygenase (KMO) and tryptophan dioxygenase (TDO), yielding the formation of toxic metabolites such as 3-OHKYN and quinolinic acid. Once 3-OHKYN crosses neuronal cell membranes through neutral amino acid transporter (NAT), it can follow two routes: (i) may act as ROS scavenger/antioxidant via direct scavenging or by triggering integral antioxidant responses evoked by transcription signaling pathways, such as the Nrf2/Keap1/ARE axis (ii) 3-OHKYN may undergoes autooxidation yielding highly reactive molecules, such as o-aminoquinone, which in turn causes exacerbated oxidative damage, activation of deadly cascades, and massive cell death.

Alternate Names

3039Hydroxykynurenine, 3-hydroxykynuenine modification

Additional 3-OHKYN Products

Product Documents for 3-OHKYN Antibody (P3UI) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for 3-OHKYN Antibody (P3UI) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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