4-1BB Ligand/TNFSF9 Antibody (JE33-91)

Western Blot: 4-1BB Ligand/TNFSF9 Antibody (JE33-91) [NBP3-31986]

Western Blot: 4-1BB Ligand/TNFSF9 Antibody (JE33-91) [NBP3-31986] - Western blot analysis of 4-1BB Ligand/TNFSF9 on different lysates with Rabbit anti-4-1BB Ligand/TNFSF9 antibody (NBP3-31986) at 1/1,000 dilution.

Lane 1: THP-1 whole cell lysate
Lane 2: THP-1 treated with 100nM TPA overnight whole cell lysate
Lane 3: HeLa cell lysate
Lane 4: HepG2 cell lysate
Lane 5: RAW264.7 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 27 kDa
Observed band size: 29 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31986) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.