4-1BB/TNFRSF9/CD137 Antibody (158332) [PE/Atto594]
Novus Biologicals, part of Bio-Techne | Catalog # FAB937PEATT594
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry
Label
PE/Atto594 (Excitation = 488 nm, Emission = 627 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 158332
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse 4-1BB
Val24-Leu187
Accession # P20334
Val24-Leu187
Accession # P20334
Specificity
Detects mouse 4-1BB in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) 4‑1BB, rhCD27, recombinant mouse (rm) CD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFas, rhGITR, rmGITR, rhHVEM, rhLTR beta, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTROY, rmTROY, rhTNF sRI, rhTNF sRII, or rmTNF sRII.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for 4-1BB/TNFRSF9/CD137 Antibody (158332) [PE/Atto594]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: 4-1BB/TNFRSF9/CD137
References
1. Etxeberria, I., Glez-Vaz, J., Teijeira, A., & Melero, I. (2020). New emerging targets in cancer immunotherapy: CD137/4-1BB costimulatory axis. ESMO open, 4(Suppl 3), e000733. https://doi.org/10.1136/esmoopen-2020-000733
2. Chester, C., Sanmamed, M. F., Wang, J., & Melero, I. (2018). Immunotherapy targeting 4-1BB: mechanistic rationale, clinical results, and future strategies. Blood, 131(1), 49-57. https://doi.org/10.1182/blood-2017-06-741041
3. Uniport (Q07011)
4. Chester, C., Ambulkar, S., & Kohrt, H. E. (2016). 4-1BB agonism: adding the accelerator to cancer immunotherapy. Cancer Immunology, Immunotherapy: CII, 65(10), 1243-1248. https://doi.org/10.1007/s00262-016-1829-2
5. Zapata, J. M., Perez-Chacon, G., Carr-Baena, P., Martinez-Forero, I., Azpilikueta, A., Otano, I., & Melero, I. (2018). CD137 (4-1BB) signalosome: complexity is a matter of TRAFs. Frontiers in Immunology, 9, 2618. https://doi.org/10.3389/fimmu.2018.02618
Alternate Names
41BB, CD137, ILA, TNFRSF9
Gene Symbol
TNFRSF9
Additional 4-1BB/TNFRSF9/CD137 Products
Product Documents for 4-1BB/TNFRSF9/CD137 Antibody (158332) [PE/Atto594]
Product Specific Notices for 4-1BB/TNFRSF9/CD137 Antibody (158332) [PE/Atto594]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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