4EBP1 Antibody (007)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89207

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-89207-50ul
NBP2-89207-100ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 007

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human 4EBP1 (Accession#: NP_004086.1; Ser2-Ile118).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for 4EBP1 Antibody (007)

Immunocytochemistry/ Immunofluorescence: 4EBP1 Antibody (007) [NBP2-89207]

Immunocytochemistry/ Immunofluorescence: 4EBP1 Antibody (007) [NBP2-89207]

Immunocytochemistry/Immunofluorescence: 4EBP1 Antibody (007) [NBP2-89207] - Staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human 4EBP1 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm.

Applications for 4EBP1 Antibody (007)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:100
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 4EBP1

4E-BP1 (eIF4E-binding protein) also known as PHAS, is a 10-12 kDa acidic protein that compete with eIF4G for binding of eiF4E to the mRNA 5' cap structure (1). Binding of the 4E-BPs to eIF4E is reversible and is dependent on the phosphorylation status of 4E-BP. Non-phosphorylated 4E-BP1 will bind strongly to eiF4E while, the phosphorylated form will no (2)t. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating 4E-BP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the 4EBP1-eIF4E interaction (3-4)

Long Name

Eukaryotic Translation Initiation Factor 4E Binding Protein 1

Alternate Names

EIF4EBP1, PHAS-I

Gene Symbol

EIF4EBP1

Additional 4EBP1 Products

Product Documents for 4EBP1 Antibody (007)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for 4EBP1 Antibody (007)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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