4EBP1 Antibody (4B6G10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47554

Novus Biologicals, part of Bio-Techne
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NBP1-47554-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4B6G10

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all 4EBP1 Antibodies »

Product Summary for 4EBP1 Antibody (4B6G10) - BSA Free

Immunogen

Purified recombinant fragment of 4EBP1 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for 4EBP1 Antibody (4B6G10) - BSA Free

Western Blot: 4EBP1 Antibody (4B6G10)BSA Free [NBP1-47554]

Western Blot: 4EBP1 Antibody (4B6G10)BSA Free [NBP1-47554]

Western Blot: 4EBP1 Antibody (4B6G10) [NBP1-47554] - Analysis using 4E-BP1 mouse mAb against truncated 4E-BP1 recombinant protein(1)and A431 cell lysate (2).
Immunohistochemistry-Paraffin: 4EBP1 Antibody (4B6G10) - BSA Free [NBP1-47554]

Immunohistochemistry-Paraffin: 4EBP1 Antibody (4B6G10) - BSA Free [NBP1-47554]

Immunohistochemistry-Paraffin: 4EBP1 Antibody (4B6G10) [NBP1-47554] - Analysis of human pancreas carcinoma (A), esophagus carcinoma tissue (B) and ovary tumor tissue (C), showing cytoplasmic and membrane localization using 4E-BP1 mouse mAb with DAB staining.

Applications for 4EBP1 Antibody (4B6G10) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 4EBP1

4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.

Long Name

Eukaryotic Translation Initiation Factor 4E Binding Protein 1

Alternate Names

EIF4EBP1, PHAS-I

Entrez Gene IDs

1978 (Human)

Gene Symbol

EIF4EBP1

Additional 4EBP1 Products

Product Documents for 4EBP1 Antibody (4B6G10) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for 4EBP1 Antibody (4B6G10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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