4EBP1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76621

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (92%), Rat (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all 4EBP1 Antibodies »

Product Summary for 4EBP1 Antibody - BSA Free

Immunogen

Antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human 4E-BP1. The immunogen is located within the last 50 amino acids of 4E-BP1. Amino Acid Squence: NSPEDKRAGGEESQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 4EBP1 Antibody - BSA Free

Western Blot: 4EBP1 AntibodyBSA Free [NBP1-76621]

Western Blot: 4EBP1 AntibodyBSA Free [NBP1-76621]

Western Blot: 4EBP1 Antibody [NBP1-76621] - eIF [NBP1-76621] - Analysis of eIF4EBP1 in human brain cell lysate with eIF4EBP1 antibody using NBP1-76621 at (A) 2.5, (B) 5 and (C) 10 ug/ml.
Immunocytochemistry/ Immunofluorescence: 4EBP1 Antibody - BSA Free [NBP1-76621]

Immunocytochemistry/ Immunofluorescence: 4EBP1 Antibody - BSA Free [NBP1-76621]

Immunocytochemistry/Immunofluorescence: 4EBP1 Antibody [NBP1-76621] - Staining of 4E-BP1 in 3T3 cells with 4E-BP1 antibody at 20 ug/ml.
Immunocytochemistry/ Immunofluorescence: 4EBP1 Antibody - BSA Free [NBP1-76621]

Immunocytochemistry/ Immunofluorescence: 4EBP1 Antibody - BSA Free [NBP1-76621]

Immunocytochemistry/Immunofluorescence: 4EBP1 Antibody [NBP1-76621] - 3T3 cells with eIF4EBP1 antibody at 2 ug/ml.

Applications for 4EBP1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

2-20 ug/ml

Western Blot

2.5-10 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 4EBP1

The translation of mRNA in eukaryotic cells is regulated by the presence of amino acids through multiple mechanisms. One such mechanism involves the evolutionarily conserved serine/threonine kinase TOR (Target of rapamycin, also known as mTOR), which regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. One downstream target of TOR is the eukaryotic initiation factor 4E binding protein 1 (4E-BP1) whose phosphorylation prevents its association with eIF4E, preferentially stimulating translation of mRNAs containing long, highly structured 5'-UTRs. Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferationl, at least in part by inhibiting the activity of TOR towards 4E-BP1.

Long Name

Eukaryotic Translation Initiation Factor 4E Binding Protein 1

Alternate Names

EIF4EBP1, PHAS-I

Gene Symbol

EIF4EBP1

UniProt

NP_004086 (Human)

Additional 4EBP1 Products

Product Documents for 4EBP1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for 4EBP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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