4EBP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16307

Novus Biologicals, part of Bio-Techne
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NBP2-16307
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (92%), Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all 4EBP1 Antibodies »

Product Specifications

Immunogen

Full length human 4EBP1 Recombinant protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 4EBP1 Antibody

Knockout Validated: 4EBP1 Antibody [NBP2-16307]

Knockout Validated: 4EBP1 Antibody [NBP2-16307]

Knockout Validated: 4EBP1 Antibody [NBP2-16307] - Wild-type (WT) and 4E-BP1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 antibody [N1C3]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: 4EBP1 Antibody [NBP2-16307]

Western Blot: 4EBP1 Antibody [NBP2-16307]

Western Blot: 4EBP1 Antibody [NBP2-16307] - 4EBP1 antibody detects 4EBP1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 antibody diluted at a dilution of 1:2000.
Immunohistochemistry-Paraffin: 4EBP1 Antibody [NBP2-16307]

Immunohistochemistry-Paraffin: 4EBP1 Antibody [NBP2-16307]

Immunohistochemistry-Paraffin: 4EBP1 Antibody [NBP2-16307] - Analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.
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Applications for 4EBP1 Antibody

Application
Recommended Usage

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: 4EBP1

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq]

Long Name

Eukaryotic Translation Initiation Factor 4E Binding Protein 1

Alternate Names

EIF4EBP1, PHAS-I

Gene Symbol

EIF4EBP1

UniProt

Q13541

Additional 4EBP1 Products

Product Documents for 4EBP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 4EBP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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