5-HT1A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21590
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Rat
Predicted:
Chimpanzee (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Simple Western, Western Blot
Cited:
Immunohistochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human 5-HT1A. The exact sequence is proprietary.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for 5-HT1A Antibody
Simple Western: 5-HT1A Antibody [NBP2-21590]
Simple Western: 5-HT1A Antibody [NBP2-21590] - Detection of 5-HT1A by Simple WesternTM. Simple Western lane view shows lysates of Raji cells. A specific band was detected for 5-HT1A at approximately 60 kDa (as indicated) using 1:100 of Rabbit Anti-Human 5-HT1A Polyclonal Antibody (Catalog # NBP2-21590). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Immunocytochemistry/ Immunofluorescence: 5-HT1A Antibody [NBP2-21590]
Immunocytochemistry/Immunofluorescence: 5-HT1A Antibody [NBP2-21590] - DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.Immunohistochemistry-Frozen: 5-HT1A Antibody [NBP2-21590]
Immunohistochemistry-Frozen: 5-HT1A Antibody [NBP2-21590] - Frozen Sectioned adult mouse hippocampus. Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal diluted at 1:250.Blue: Fluoroshield with DAPI.Applications for 5-HT1A Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry-Frozen
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: 5-HT1A
Long Name
5-Hydroxytryptamine Receptor 1A
Alternate Names
5HT1A, ADRB2RL1, ADRBRL1, G-21, HTR1A, PFMCD
Gene Symbol
HTR1A
UniProt
Additional 5-HT1A Products
Product Documents for 5-HT1A Antibody
Product Specific Notices for 5-HT1A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.