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5-HT1A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21590

Novus Biologicals, part of Bio-Techne
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NBP2-21590

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Predicted:

Chimpanzee (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Simple Western, Western Blot

Cited:

Immunohistochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human 5-HT1A. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 5-HT1A Antibody

Simple Western: 5-HT1A Antibody [NBP2-21590]

Simple Western: 5-HT1A Antibody [NBP2-21590]

Simple Western: 5-HT1A Antibody [NBP2-21590] - Detection of 5-HT1A by Simple WesternTM. Simple Western lane view shows lysates of Raji cells. A specific band was detected for 5-HT1A at approximately 60 kDa (as indicated) using 1:100 of Rabbit Anti-Human 5-HT1A Polyclonal Antibody (Catalog # NBP2-21590). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Immunocytochemistry/ Immunofluorescence: 5-HT1A Antibody [NBP2-21590]

Immunocytochemistry/ Immunofluorescence: 5-HT1A Antibody [NBP2-21590]

Immunocytochemistry/Immunofluorescence: 5-HT1A Antibody [NBP2-21590] - DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Frozen: 5-HT1A Antibody [NBP2-21590]

Immunohistochemistry-Frozen: 5-HT1A Antibody [NBP2-21590]

Immunohistochemistry-Frozen: 5-HT1A Antibody [NBP2-21590] - Frozen Sectioned adult mouse hippocampus. Green: 5-HT1A receptor protein stained by 5-HT1A receptor antibody [N3C1], Internal diluted at 1:250.Blue: Fluoroshield with DAPI.

Applications for 5-HT1A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: 5-HT1A

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.

Long Name

5-Hydroxytryptamine Receptor 1A

Alternate Names

5HT1A, ADRB2RL1, ADRBRL1, G-21, HTR1A, PFMCD

Gene Symbol

HTR1A

UniProt

Additional 5-HT1A Products

Product Documents for 5-HT1A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 5-HT1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.