5-HT6 Antibody

Immunohistochemistry: 5-HT6 Antibody [NBP1-46557]

Immunohistochemistry: 5-HT6 Antibody [NBP1-46557] - LSM image showing strong expression of 5-HT6 (red) colocalized with virally transduced (green) TH (blue)-positive neurons within the dopaminergic fetal graft. Image collected and cropped by CiteAb from the following publication (linkinghub.elsevier.com/retrieve/pii/S0896627316300769), licensed under a CC-BY license.

Immunohistochemistry: 5-HT6 Antibody [NBP1-46557]

Immunohistochemistry: 5-HT6 Antibody [NBP1-46557] - The 5-HT6 Receptor Is highly expressed in human tissue grafts originating from both fetal and hESC. Immunofluorescence staining of 5-HT6 expression in a human dopaminergic fetal graft within the rat striatum with the phenotypic staining for TH , and the identification of human NCAM, imaged using LSM. Image collected and cropped by CiteAb from the following publication (linkinghub.elsevier.com/retrieve/pii/S0896627316300769), licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: 5-HT6 Antibody [NBP1-46557] -

Immunocytochemistry/ Immunofluorescence: 5-HT6 Antibody [NBP1-46557] - The 5-HT6 Receptor Is Highly Expressed in Human Tissue Grafts Originating from Both Fetal & hESC Sources(A–E) Immunofluorescence staining of 5-HT6 expression in a human dopaminergic fetal graft within the rat striatum (A & C) with the phenotypic staining for TH (A & B), & the identification of human NCAM (A & D), imaged using LSM. The 5-HT6 expression was confirmed to reside in DA neurons originating from the transplanted tissue (E).(F–J) Similarly, immunofluorescence staining of a paraffin-embedded section originating from the putamen of a PD patient that received a dopaminergic fetal graft, imaged using LSM, confirmed that the 5-HT6 receptor (F & H) is highly expressed in the TH-positive (F & G, with overlay in J) DA neurons originating from the fetal graft. Nuclear staining (DAPI) confirmed the 5-HT6 expression to be abundant in the neuronal soma (I).(K–O) To confirm if this receptor is also abundant in DA neurons differentiated from hESCs, a graft derived from the H9 hESC line, differentiated using the novel floor-plate-based protocol & transplanted to the parkinsonian striatum of a nude rat, was evaluated using LSM with immunofluorescence for the same genes: 5-HT6 (K & M), TH (K & L), & the human NCAM (K & N). This confirmed that this receptor is highly expressed in DA neurons also differentiated from this cell source, when transplanted to the striatum. Overlay of all three antigens confirmed the 5-HT6 receptor to reside in hESC-derived DA neurons (O). Scale bar, 100 μm (K) & 50 μm (N). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27161524), licensed under a CC-BY license. Not internally tested by Novus Biologicals.