5-Lipoxygenase Antibody (1D4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15171

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15171
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1D4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all 5-Lipoxygenase Antibodies »

Product Summary for 5-Lipoxygenase Antibody (1D4)

Immunogen

A synthesized peptide derived from human 5-Lipoxygenase. UniProt ID (P09917).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 5-Lipoxygenase Antibody (1D4)

Immunohistochemistry: 5-Lipoxygenase Antibody (1D4) [NBP3-15171]

Immunohistochemistry: 5-Lipoxygenase Antibody (1D4) [NBP3-15171]

Immunohistochemistry: 5-Lipoxygenase Antibody (1D4) [NBP3-15171] - IHC image of 5-Lipoxygenase antibody diluted at 1:100 and staining in paraffin-embedded human lung tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: 5-Lipoxygenase Antibody (1D4) [NBP3-15171]

Immunohistochemistry: 5-Lipoxygenase Antibody (1D4) [NBP3-15171]

Immunohistochemistry: 5-Lipoxygenase Antibody (1D4) [NBP3-15171] - IHC image of 5-Lipoxygenase antibody diluted at 1:100 and staining in paraffin-embedded human spleen tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for 5-Lipoxygenase Antibody (1D4)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: 5-Lipoxygenase

5 Lipoxygenase encodes a protein which, with Arachidonate 5-lipoxygenase-activating protein, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.

Alternate Names

5Lipoxygenase, 5LPG, ALOX5, LOG5, LOX5A

Gene Symbol

ALOX5

Additional 5-Lipoxygenase Products

Product Documents for 5-Lipoxygenase Antibody (1D4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for 5-Lipoxygenase Antibody (1D4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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