5-MethylCytosine Antibody (C.15200006)

Immunofluorescence: 5-MethylCytosine Antibody (C.15200006) [NBP2-59170]

Immunofluorescence: 5-MethylCytosine Antibody (C.15200006) [NBP2-59170] - HeLa cells were stained with the antibody against 5-mC and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 1% BSA. The cells were immunofluorescently labelled with the 5-mC antibody (left) diluted 1:1,000 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Methylated DNA Immunoprecipitation: 5-MethylCytosine Antibody (C.15200006) [NBP2-59170]

Methylated DNA Immunoprecipitation: 5-MethylCytosine Antibody (C.15200006) [NBP2-59170] - Methylated DNA immunoprecipitation was performed on 1 ug fragmented human genomic DNA using 0.2 ug of the antibody against 5-mC and a MeDIP Kit. The fragmented DNA was spiked with the internal controls present in the kit (methylated DNA (meDNA) as a positive and unmethylated DNA (unDNA) as a negative control) prior to performing the IP. QPCR was performed with optimized primer sets, specific for the methylated and unmethylated DNA controls, and for a known methylated (TSH2B) and unmethylated (GAPDH) genomic region. Figure shows the recovery expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).