5'-Nucleotidase/CD73 Antibody (001)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89705

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 001

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all 5'-Nucleotidase/CD73 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human 5'-Nucleotidase/CD73 (Accession#: NP_002517.1; Met1-Lys547).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for 5'-Nucleotidase/CD73 Antibody (001)

Western Blot: 5'-Nucleotidase/CD73 Antibody (001) [NBP2-89705]

Western Blot: 5'-Nucleotidase/CD73 Antibody (001) [NBP2-89705]

Western Blot: 5'-Nucleotidase/CD73 Antibody (001) [NBP2-89705] - Lane A: NCI-H1299 Whole Cell Lysate Lane B: HepG2 Whole Cell LysateLane C: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:63 kDa Observed band size:70 kDa (We are unsure as to the identity of these extra bands.)
Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (001) [NBP2-89705]

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (001) [NBP2-89705]

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (001) [NBP2-89705] - Staining of human NT5E in human spleen with rabbit monoclonal antibody (1:5000).

Applications for 5'-Nucleotidase/CD73 Antibody (001)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:2500-1:10000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 5'-Nucleotidase/CD73

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Alternate Names

5-NT, CD73, E5NT, eNT, NT5E, NTE

Gene Symbol

NT5E

Additional 5'-Nucleotidase/CD73 Products

Product Documents for 5'-Nucleotidase/CD73 Antibody (001)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for 5'-Nucleotidase/CD73 Antibody (001)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.