Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (95%), Mouse (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 45M4F9

Format

Azide Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all 5'-Nucleotidase/CD73 Antibodies »

Product Specifications

Immunogen

Within the range of amino acids 169-324 of human CD73 were used as the immunogen for the antibody.

Specificity

This product can detect Isoform 1 and Isoform of Human CD73.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for 5'-Nucleotidase/CD73 Antibody (45M4F9) - Azide Free

Western Blot: 5'-Nucleotidase/CD73 Antibody (45M4F9)Azide Free [NBP2-25243]

Western Blot: 5'-Nucleotidase/CD73 Antibody (45M4F9)Azide Free [NBP2-25243]

Western Blot: 5'-Nucleotidase/CD73 Antibody (45M4F9) - Azide Free [NBP2-25243] - analysis of CD73 in a partial recombinant protein using CD73 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (45M4F9) - Azide Free [NBP2-25243]

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (45M4F9) - Azide Free [NBP2-25243]

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (45M4F9) - Azide Free [NBP2-25243] - Formalin-fixed, paraffin-embedded human breast stained with CD73 antibody (20 ug/ml), peroxidase-conjugate and DAB chromogen.

Applications for 5'-Nucleotidase/CD73 Antibody (45M4F9) - Azide Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

2-5 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 ml sterile PBS

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 5'-Nucleotidase/CD73

CD73 is a membrane-bound enzyme having a key role in extracellular ATP metabolism producing adenosine by dephosphorylating AMP. It is highly expressed on endothelial cells but a moderate expression is also observed on a subpopulation of lymphocytes, especially regulatory T cells. Although CD73 on the endothelial cells and lymphocytes are structurally similar, they are differently regulated. Triggering of lymphocyte CD73 with antibodies results in cleavage of CD73 and activation of LFA-1, leading to increased adhesion of lymphocytes to endothelium (Algars A et al., 2011). CD73 plays an important role in carcinogenesis, as adenosine promotes tumor progression and CD73-expressing cancer cell lines are more aggressive (Supernat A et al., 2012). Extracellular adenosine is a potent immunosuppressor that accumulates during tumor growth. Anti-CD73 monoclonal antibody therapy significantly delays primary 4T1.2 and E0771 tumor growth in immune-competent mice and significantly inhibits the development of spontaneous 4T1.2 lung metastases. Antibody therapy is essentially dependent on the induction of adaptive anti-tumor immune responses (Stagg J et al., 2010).

Alternate Names

5-NT, CD73, E5NT, eNT, NT5E, NTE

Gene Symbol

NT5E

Additional 5'-Nucleotidase/CD73 Products

Product Documents for 5'-Nucleotidase/CD73 Antibody (45M4F9) - Azide Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for 5'-Nucleotidase/CD73 Antibody (45M4F9) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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