5'-Nucleotidase/CD73 Antibody (4G6B10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37274

Novus Biologicals, part of Bio-Techne
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NBP2-37274
NBP2-37274-0.025ml
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4G6B10

Format

BSA Free

Concentration

1 mg/ml

View all 5'-Nucleotidase/CD73 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human 5'-Nucleotidase/CD73 (AA: 30-250) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

63.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 5'-Nucleotidase/CD73 Antibody (4G6B10) - BSA Free

Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10)BSA Free [NBP2-37274]

Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10)BSA Free [NBP2-37274]

Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mouse mAb against A431 (1) cell lysate.
Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6B10) - BSA Free [NBP2-37274]

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6B10) - BSA Free [NBP2-37274]

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis of esophagus cancer tissues using NT5E mouse mAb with DAB staining.
Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10)BSA Free [NBP2-37274]

Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10)BSA Free [NBP2-37274]

Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6B10) [NBP2-37274] - Analysis using NT5E mAb against human NT5E (AA: ) recombinant protein. (Expected MW is 26.6 kDa)
View 3 more images

Applications for 5'-Nucleotidase/CD73 Antibody (4G6B10) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 5'-Nucleotidase/CD73

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Alternate Names

5-NT, CD73, E5NT, eNT, NT5E, NTE

Gene Symbol

NT5E

Additional 5'-Nucleotidase/CD73 Products

Product Documents for 5'-Nucleotidase/CD73 Antibody (4G6B10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for 5'-Nucleotidase/CD73 Antibody (4G6B10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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