5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48480

Clone AD2 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP2-48480
NBP2-48480SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow (Cell Surface), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Cited:

Flow Cytometry, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # AD2

Format

BSA Free

Concentration

1.0 mg/ml

View all 5'-Nucleotidase/CD73 Antibodies »

Product Specifications

Immunogen

Human CD73 [UniProt# P21589]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free [NBP2-48480]

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free [NBP2-48480]

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - Analysis of 5'-Nucleotidase/CD73 antibody on Human lung tissue. PFA fixed paraffin-embedded sections. Sections were incubated with the primary antibody overnight in 4C (1:200 dilution). Image from verified customer review.
Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free [NBP2-48480]

Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free [NBP2-48480]

Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on CD3+ human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25186PCP.
Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free [NBP2-48480]

Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free [NBP2-48480]

Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) [NBP2-48480] - A surface stain was performed on U87 MG cells with 5'-Nucleotidase/CD73 Antibody (AD2) antibody NBP2-48480 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.
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Applications for 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:500

Immunohistochemistry-Frozen

reported in scientific literature (PMID 1989648)

Immunohistochemistry-Paraffin

reported by customer review
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-48480 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 5'-Nucleotidase/CD73

This AD2 monoclonal CD73 antibody reacts with human CD73, a 5'-ectonucleotidase that converts 5'-adenosine monophosphate to adenosine. CD73 is expressed on the surface of endothelial cells, as well as B and T cells, including some CD4+Foxp3+ regulatory T cells. Adenosine production by these cells has been linked to the inhibition of CD4 T cell effector functions such as proliferation and cytokine secretion.

Alternate Names

5-NT, CD73, E5NT, eNT, NT5E, NTE, ad2 anti cd73, ad2 cd73

Gene Symbol

NT5E

Additional 5'-Nucleotidase/CD73 Products

Product Documents for 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for 5'-Nucleotidase/CD73 Antibody (AD2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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