5'-Nucleotidase/CD73 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32951
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Predicted:
Bovine (91%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human 5'-Nucleotidase/CD73. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%).
Localization
Cell membrane, GPI-anchor
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for 5'-Nucleotidase/CD73 Antibody
![Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP1-32951]](https://resources.bio-techne.com/images/products/5'-Nucleotidase-CD73-Antibody-Western-Blot-NBP1-32951-img0006.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP1-32951]")
Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP1-32951]
Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP1-32951] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-32951]](https://resources.bio-techne.com/images/products/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-Paraffin-NBP1-32951-img0005.jpg "Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-32951]")
Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-32951]
Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-32951] - MDAMB231 xenograft, using CD73 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP1-32951] -
Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP1-32951] - Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for 5'-Nucleotidase/CD73 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: 5'-Nucleotidase/CD73
Alternate Names
5-NT, CD73, E5NT, eNT, NT5E, NTE
Entrez Gene IDs
4907 (Human)
Gene Symbol
NT5E
UniProt
Additional 5'-Nucleotidase/CD73 Products
- All Products for 5'-Nucleotidase/CD73
- 5'-Nucleotidase/CD73 Activity Assays
- 5'-Nucleotidase/CD73 cDNA Clones
- 5'-Nucleotidase/CD73 ELISA Kits
- 5'-Nucleotidase/CD73 Lysates
- 5'-Nucleotidase/CD73 Primary Antibodies
- 5'-Nucleotidase/CD73 Proteins and Enzymes
- 5'-Nucleotidase/CD73 Small Molecules and Peptides
Product Documents for 5'-Nucleotidase/CD73 Antibody
Product Specific Notices for 5'-Nucleotidase/CD73 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.