5T4 Antibody (1B6)
Novus Biologicals, part of Bio-Techne | Catalog # H00007162-M09
Key Product Details
Species Reactivity
Human, Rat
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 1B6
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
TPBG (NP_006661, 219 a.a. ~ 328 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SNHFLYLPRDVLAQLPSLRHLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHIRVFLDNNPWVCDCHMADMVTWLKETEVVQGKDRLTCAYPEK
Specificity
TPBG - trophoblast glycoprotein, (1B6)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for 5T4 Antibody (1B6)
![Western Blot: 5T4 Antibody (1B6) [H00007162-M09]](https://resources.bio-techne.com/images/products/5T4-Antibody-1B6-Western-Blot-H00007162-M09-img0006.jpg "Western Blot: 5T4 Antibody (1B6) [H00007162-M09]")
Western Blot: 5T4 Antibody (1B6) [H00007162-M09]
Western Blot: 5T4 Antibody (1B6) [H00007162-M09] - Analysis of TPBG expression in HeLa (Cat # L013V1).![Western Blot: 5T4 Antibody (1B6) [H00007162-M09]](https://resources.bio-techne.com/images/products/5T4-Antibody-1B6-Western-Blot-H00007162-M09-img0007.jpg "Western Blot: 5T4 Antibody (1B6) [H00007162-M09]")
Western Blot: 5T4 Antibody (1B6) [H00007162-M09]
Western Blot: 5T4 Antibody (1B6) [H00007162-M09] - Analysis of TPBG expression in PC-12 (Cat # L012V1).
Sandwich ELISA: 5T4 Antibody (1B6) [H00007162-M09] - Detection limit for recombinant GST tagged TPBG is approximately 1ng/ml as a capture antibody.
Applications for 5T4 Antibody (1B6)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate for WB. It has been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: 5T4
Long Name
Oncofetal Antigen/Trophoblast Glycoprotein
Alternate Names
M6P1, TPBG
Entrez Gene IDs
7162 (Human)
Gene Symbol
TPBG
UniProt
Additional 5T4 Products
Product Documents for 5T4 Antibody (1B6)
Product Specific Notices for 5T4 Antibody (1B6)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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