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6 Phosphofructo 2 Kinase Antibody (7X10D9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16295

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-16295-100ul
NBP3-16295-20ul

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7X10D9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 400-500 of human 6 Phosphofructo 2 Kinase (NP_004557.1). LDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVCTHRERSEDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPT

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 6 Phosphofructo 2 Kinase Antibody (7X10D9)

Western Blot: 6 Phosphofructo 2 Kinase Antibody (7X10D9) [NBP3-16295]

Western Blot: 6 Phosphofructo 2 Kinase Antibody (7X10D9) [NBP3-16295]

Western Blot: 6 Phosphofructo 2 Kinase Antibody (7X2H3) [NBP3-16295] - Western blot analysis of extracts of various cell lines, using 6 Phosphofructo 2 Kinase Rabbit mAb (NBP3-16295) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunocytochemistry/ Immunofluorescence: 6 Phosphofructo 2 Kinase Antibody (7X10D9) [NBP3-16295]

Immunocytochemistry/ Immunofluorescence: 6 Phosphofructo 2 Kinase Antibody (7X10D9) [NBP3-16295]

Immunocytochemistry/Immunofluorescence: 6 Phosphofructo 2 Kinase Antibody (7X2H3) [NBP3-16295] - Immunofluorescence analysis of PC-12 cells using 6 Phosphofructo 2 Kinase Rabbit mAb (NBP3-16295) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: 6 Phosphofructo 2 Kinase Antibody (7X10D9) [NBP3-16295]

Immunohistochemistry-Paraffin: 6 Phosphofructo 2 Kinase Antibody (7X10D9) [NBP3-16295]

Immunohistochemistry-Paraffin: 6 Phosphofructo 2 Kinase Antibody (7X2H3) [NBP3-16295] - Rat ovary using PFKFB3 Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for 6 Phosphofructo 2 Kinase Antibody (7X10D9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 -1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PFKFB3

Synthesis and degradation of fructose 2,6-bisphosphate

Long Name

6-Phosphofructo-2-Kinase/ Fructose-2,6-Bisphosphatase

Alternate Names

6 Phosphofructo 2 Kinase, IPFK2, PFK/FBPase 3, PFK2

Gene Symbol

PFKFB3

Additional PFKFB3 Products

Product Documents for 6 Phosphofructo 2 Kinase Antibody (7X10D9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 6 Phosphofructo 2 Kinase Antibody (7X10D9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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