Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PFKFB3 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 470 to 520 of human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 using the numbering given in entry NP_004557.1 (GeneID 5209).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 6 Phosphofructo 2 Kinase Antibody

Western Blot: 6 Phosphofructo 2 Kinase Antibody [NBP2-32163]

Western Blot: 6 Phosphofructo 2 Kinase Antibody [NBP2-32163]

Western Blot: 6 Phosphofructo 2 Kinase Antibody [NBP2-32163] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-6 Phosphofructo 2 Kinase antibody NBP2-32163 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: 6 Phosphofructo 2 Kinase Antibody [NBP2-32163]

Immunoprecipitation: 6 Phosphofructo 2 Kinase Antibody [NBP2-32163]

Immunoprecipitation: 6 Phosphofructo 2 Kinase Antibody [NBP2-32163] - Samples: Whole cell lysate (0.5 to 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-6 Phosphofructo 2 Kinase antibody NBP2-32163 used for IP at 6 ug per reaction. For blotting immunoprecipitated 6 Phosphofructo 2 Kinase, NBP2-32163 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for 6 Phosphofructo 2 Kinase Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PFKFB3

Synthesis and degradation of fructose 2,6-bisphosphate

Long Name

6-Phosphofructo-2-Kinase/ Fructose-2,6-Bisphosphatase

Alternate Names

6 Phosphofructo 2 Kinase, IPFK2, PFK/FBPase 3, PFK2

Gene Symbol

PFKFB3

UniProt

NP_004557.1 (Human)

Additional PFKFB3 Products

Product Documents for 6 Phosphofructo 2 Kinase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 6 Phosphofructo 2 Kinase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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