A-RAF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55054

Novus Biologicals, part of Bio-Techne
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NBP2-55054
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all A-Raf Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: DSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPAEQRERKSLADDKKKVKNLGYRDS

Reactivity Notes

Mouse 86%, Rat 88%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for A-RAF Antibody

Western Blot: A-RAF Antibody [NBP2-55054]

Western Blot: A-RAF Antibody [NBP2-55054]

Western Blot: A-RAF Antibody [NBP2-55054] - Analysis using Anti-ARAF antibody NBP2-55054 (A) shows similar pattern to independent antibody NBP2-49479 (B).
Immunocytochemistry/ Immunofluorescence: A-RAF Antibody [NBP2-55054]

Immunocytochemistry/ Immunofluorescence: A-RAF Antibody [NBP2-55054]

Immunocytochemistry/Immunofluorescence: A-RAF Antibody [NBP2-55054] - Staining of human cell line HEK 293 shows localization to nucleus & nucleoli fibrillar center.

Applications for A-RAF Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: A-Raf

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.

Long Name

v-Raf Murine Sarcoma 3611 Viral Oncogene Homolog

Alternate Names

ARaf, PKS, PKS2, RAFA1

Gene Symbol

ARAF

Additional A-Raf Products

Product Documents for A-RAF Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for A-RAF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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