A2BP1 Antibody (D8H8) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80553
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # D8H8
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Mouse recombinant A2BP1 [Swiss-Prot# Q9JJ43]
Localization
Nucleus. Cytoplasm. Note: Predominantly nuclear.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for A2BP1 Antibody (D8H8) - Azide and BSA Free
Western Blot: A2BP1 Antibody (D8H8)Azide and BSA Free [NBP2-80553]
Western Blot: A2BP1 Antibody (D8H8) - Azide and BSA Free [NBP2-80553] - Western blot analysis of A2BP1 expression in 1) human brain and 2) mouse brain tissue lysates. Image from the standard format of this antibody.Immunocytochemistry/ Immunofluorescence: A2BP1 Antibody (D8H8) - Azide and BSA Free [NBP2-80553]
Immunocytochemistry/Immunofluorescence: A2BP1 Antibody (D8H8) - Azide and BSA Free [NBP2-80553] - A2BP1 antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image from the standard format of this antibody.Immunohistochemistry: A2BP1 Antibody (D8H8) - Azide and BSA Free [NBP2-80553]
Immunohistochemistry: A2BP1 Antibody (D8H8) - Azide and BSA Free [NBP2-80553] - IHC staining of A2BP1 in mouse brain using DAB with hematoxylin counterstain. Image from the standard format of this antibody.Applications for A2BP1 Antibody (D8H8) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Immunohistochemistry
1:200
Immunohistochemistry-Frozen
reported in scientific literature (PMID 30001398)
Immunohistochemistry-Paraffin
1:200
Immunoprecipitation
1:10-1:100
Simple Western
1:20
Western Blot
1:1000
Application Notes
In Western Blot, a band is seen 50-55 kDa representing A2BP1. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
Tris-Glycine (pH 7.5), 0.15M NaCl
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: A2BP1
Alternate Names
2BP1, A2BP, A2BP1 Hexaribonucleotide-binding protein 1, Ataxin-2-binding protein 1, FOX-1, FOX1 hexaribonucleotide binding protein 1, Fox-1 homolog A, HRNBP1 ataxin 2-binding protein 1, RNA binding protein fox-1 homolog 1, RNA binding protein, fox-1 homolog (C. elegans) 1
Gene Symbol
RBFOX1
Additional A2BP1 Products
Product Documents for A2BP1 Antibody (D8H8) - Azide and BSA Free
Product Specific Notices for A2BP1 Antibody (D8H8) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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