AADACL1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76805
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Canine (100%), Mouse (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-RTRNSYIKWLDQN, from the C Terminus of the protein sequence according to NP_065843.3; NP_001139748.2; NP_001139749.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for AADACL1 Antibody
![Flow Cytometry: AADACL1 Antibody [NBP2-76805]](https://resources.bio-techne.com/images/products/AADACL1-Antibody-Flow-Cytometry-NBP2-76805-img0001.jpg "Flow Cytometry: AADACL1 Antibody [NBP2-76805]")
Flow Cytometry: AADACL1 Antibody [NBP2-76805]
Flow Cytometry: AADACL1 Antibody [NBP2-76805] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.![Immunofluorescence: AADACL1 Antibody [NBP2-76805]](https://resources.bio-techne.com/images/products/AADACL1-Antibody-Immunofluorescence-NBP2-76805-img0002.jpg "Immunofluorescence: AADACL1 Antibody [NBP2-76805]")
Immunofluorescence: AADACL1 Antibody [NBP2-76805]
Immunofluorescence: AADACL1 Antibody [NBP2-76805] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).![Immunofluorescence: AADACL1 Antibody [NBP2-76805]](https://resources.bio-techne.com/images/products/AADACL1-Antibody-Immunofluorescence-NBP2-76805-img0003.jpg "Immunofluorescence: AADACL1 Antibody [NBP2-76805]")
Immunofluorescence: AADACL1 Antibody [NBP2-76805]
Immunofluorescence: AADACL1 Antibody [NBP2-76805] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).Applications for AADACL1 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/ml
Peptide ELISA
Detection Limit 1:1000
Application Notes
WB: Preliminary experiments gave an approx. 26-28 kDa band in human brain (amygdala, cerebellum, hippocampus) lysates after 1 ug/ml antibody staining. The 26-28 kDa band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 49.1 kDa band according to NP_065843.3 and 31.2 kDa according to NP_001139749.1.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: AADACL1
Long Name
Neutral cholesterol ester hydrolase 1
Alternate Names
EC 3.1.1.-, EC 3.1.1.71, KIAA1363, NCEH, NCEH1
Gene Symbol
NCEH1
Additional AADACL1 Products
Product Specific Notices for AADACL1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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