ABCA1 Antibody (HJ1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2068
![Western Blot: ABCA1 Antibody (HJ1)BSA Free [NB100-2068]](https://resources.bio-techne.com/images/products/ABCA1-Antibody-HJ1-Western-Blot-NB100-2068-img0012.jpg "Western Blot: ABCA1 Antibody (HJ1)BSA Free [NB100-2068]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat, Rabbit
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # HJ1
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
50 kDa N-terminal extracellular loop of mouse ABCA1 Antibody (HJ1) UniProt# P41233]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ABCA1 Antibody (HJ1) - BSA Free
![Immunohistochemistry: ABCA1 Antibody (HJ1) - BSA Free [NB100-2068]](https://resources.bio-techne.com/images/products/ABCA1-Antibody-HJ1-Immunohistochemistry-NB100-2068-img0006.jpg "Immunohistochemistry: ABCA1 Antibody (HJ1) - BSA Free [NB100-2068]")
Immunohistochemistry: ABCA1 Antibody (HJ1) - BSA Free [NB100-2068]
Immunohistochemistry: ABCA1 Antibody (HJ1) [NB100-2068] - Analysis of ABCA1 in human renal cancer using DAB with hematoxylin counterstain.![Flow Cytometry: ABCA1 Antibody (HJ1) - BSA Free [NB100-2068]](https://resources.bio-techne.com/images/products/ABCA1-Antibody-HJ1-Flow-Cytometry-NB100-2068-img0010.jpg "Flow Cytometry: ABCA1 Antibody (HJ1) - BSA Free [NB100-2068]")
Flow Cytometry: ABCA1 Antibody (HJ1) - BSA Free [NB100-2068]
Flow Cytometry: ABCA1 Antibody (HJ1) [NB100-2068] - Staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using ABCA1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.Applications for ABCA1 Antibody (HJ1) - BSA Free
Application
Recommended Usage
Flow Cytometry
1 ug per million cells. Use reported in scientific literature
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 31061090)
Immunohistochemistry
1:400
Immunohistochemistry-Paraffin
1:400
Western Blot
1:2000
Application Notes
In Western blot a band is seen ~250 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ABCA1
ABCA1 is comprised of 2,261 amino acids with a theoretical molecular weight of 254 kDa and human ABCA1 shares 97% amino acid identity with mouse ABCA1. The general structure of ABCA consists of two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs). ABCA1 is a widely distributed cell-membrane protein with the highest expression found in macrophages. DHHC8 mediated palmitoylation of ABCA1 is essential for its localization to the plasma membrane and expression of mouse ABCA1 (not human) is induced by cAMP analogs (2). ABCA1 is phosphorylated at Ser1042 and Ser2054 by PKA, with Ser2054 being key for regulating phospholipid efflux. Mutations in ABCA1 have been linked to atherosclerosis and the progression of metabolic syndrome phenotypes: high density lipoprotein deficiency type 1 (HDLD1); also known as Tangier disease (TGD), and high density lipoprotein deficiency type 2 (HDLD2); also known as familial hypoalphalipoproteinemia (FHA) (3).
References
1.Oram JF, Lawn RM. (2001) ABCA1. The gatekeeper for eliminating excess tissue cholesterol. J Lipid Res. 42(8):1173-9. PMID: 11483617
2.Singaraja RR, Kang MH, Vaid K, Sanders SS, Vilas GL, Arstikaitis P, Coutinho J, Drisdel RC, El-Husseini Ael D, Green WN, Berthiaume L, Hayden MR. (2009) Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function. Circ Res. 105(2):138-47. PMID: 19556522
3.Attie AD. (2007) ABCA1: at the nexus of cholesterol, HDL and atherosclerosis. Trends in Biochemical Sciences 32(4):172-9. PMID: 17324574
Long Name
ATP-binding Cassette, Sub-family A (ABC1), Member 1
Alternate Names
ABC1, CERP, HDLDT1, TGD
Gene Symbol
ABCA1
Additional ABCA1 Products
Product Documents for ABCA1 Antibody (HJ1) - BSA Free
Product Specific Notices for ABCA1 Antibody (HJ1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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