ABCE1 Antibody (1N4I7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16763
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1N4I7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 500-599 of human ABCE1 ( NP_002931.2). AARVVKRFILHAKKTAFVVEHDFIMATYLADRVIVFDGVPSKNTVANSPQTLLAGMNKFLSQLEITFRRDPNNYRPRINKLNSIKDVEQKKSGNYFFLDD
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ABCE1 Antibody (1N4I7)
![Western Blot: ABCE1 Antibody (1N4I7) [NBP3-16763]](https://resources.bio-techne.com/images/products/ABCE1-Antibody-1N4I7-Western-Blot-NBP3-16763-img0002.jpg "Western Blot: ABCE1 Antibody (1N4I7) [NBP3-16763]")
Western Blot: ABCE1 Antibody (1N4I7) [NBP3-16763]
Western Blot: ABCE1 Antibody (1N4I7) [NBP3-16763] - Western blot analysis of extracts of various cell lines, using ABCE1 Rabbit mAb (NBP3-16763) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.![Immunocytochemistry/ Immunofluorescence: ABCE1 Antibody (1N4I7) [NBP3-16763]](https://resources.bio-techne.com/images/products/ABCE1-Antibody-1N4I7-Immunocytochemistry-Immunofluorescence-NBP3-16763-img0001.jpg "Immunocytochemistry/ Immunofluorescence: ABCE1 Antibody (1N4I7) [NBP3-16763]")
Immunocytochemistry/ Immunofluorescence: ABCE1 Antibody (1N4I7) [NBP3-16763]
Immunocytochemistry/Immunofluorescence: ABCE1 Antibody (1N4I7) [NBP3-16763] - Immunofluorescence analysis of NIH-3T3 cells using ABCE1 Rabbit mAb (NBP3-16763) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for ABCE1 Antibody (1N4I7)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ABCE1
ABCE-1 interacts with RNASEL to inhibit its endoribonuclease activity, and antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. ABCE1 is activated by encephalomyocarditis virus (EMCV) and HIV-1, and recently it has been found to be essential for the assembly of immature immunodeficiency virus capsids.
ABCE1 antibodies are useful tools for HIV studies and research on the ATP-binding cassette families.
Alternate Names
2'-5'-oligoadenylate-binding protein, ATP-binding cassette, sub-family E (OABP), member 1, huHP68, OABP, Ribonuclease 4 inhibitor, ribonuclease L (2'-5'-oligoisoadenylate synthetase-dependent) inhibitor, RLIABC38, RNase L inhibitor, RNASEL1, RNASELIRNS4IATP-binding cassette sub-family E member 1
Gene Symbol
ABCE1
Additional ABCE1 Products
Product Documents for ABCE1 Antibody (1N4I7)
Product Specific Notices for ABCE1 Antibody (1N4I7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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