ABH1 Antibody (5K4W5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16787
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5K4W5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for ABH1 Antibody (5K4W5)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ABH1 (Q13686). MGKMAAAVGSVATLATEPGEDAFRKLFRFYRQSRPGTADLEGVIDFSAAHAARGKGPGAQKVIKSQLNVSSVSEQNAYRAGLQPVSKWQAYGLKGYPGFI
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ABH1 Antibody (5K4W5)
![Immunohistochemistry-Paraffin: ABH1 Antibody (5K4W5) [NBP3-16787]](https://resources.bio-techne.com/images/products/ABH1-Antibody-5K4W5-Immunohistochemistry-Paraffin-NBP3-16787-img0002.jpg "Immunohistochemistry-Paraffin: ABH1 Antibody (5K4W5) [NBP3-16787]")
Immunohistochemistry-Paraffin: ABH1 Antibody (5K4W5) [NBP3-16787]
Immunohistochemistry-Paraffin: ABH1 Antibody (5K4W5) [NBP3-16787] - Immunohistochemistry of paraffin-embedded rat kidney using ABH1 Rabbit mAb (NBP3-16787) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.![Immunohistochemistry-Paraffin: ABH1 Antibody (5K4W5) [NBP3-16787]](https://resources.bio-techne.com/images/products/ABH1-Antibody-5K4W5-Immunohistochemistry-Paraffin-NBP3-16787-img0001.jpg "Immunohistochemistry-Paraffin: ABH1 Antibody (5K4W5) [NBP3-16787]")
Immunohistochemistry-Paraffin: ABH1 Antibody (5K4W5) [NBP3-16787]
Immunohistochemistry-Paraffin: ABH1 Antibody (5K4W5) [NBP3-16787] - Immunohistochemistry of paraffin-embedded mouse brain using ABH1 Rabbit mAb (NBP3-16787) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Western Blot: ABH1 Antibody (5K4W5) [NBP3-16787] -
Western Blot: ABH1 Antibody (5K4W5) [NBP3-16787] -Analysis of various lysates, using ALKBH1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.Applications for ABH1 Antibody (5K4W5)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ABH1
Alternate Names
ABH1, ABHalkB, alkylation repair homolog (E. coli), ALKB, alkB, alkylation repair homolog 1 (E. coli), ALKBH, alkylated DNA repair protein alkB homolog 1, alkylation repair, alkB homolog, Alpha-ketoglutarate-dependent dioxygenase ABH1, DNA lyase ABH1, EC 1.14.11.-, EC 4.2.99.18, hABH
Gene Symbol
ALKBH1
Additional ABH1 Products
Product Documents for ABH1 Antibody (5K4W5)
Product Specific Notices for ABH1 Antibody (5K4W5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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