ABHD14B Antibody (011)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90260
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 011
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for ABHD14B Antibody (011)
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human ABHD14B (Uniprot#: Q96IU4-1; Met1-Gln210).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for ABHD14B Antibody (011)
Western Blot: ABHD14B Antibody (011) [NBP2-90260]
Western Blot: ABHD14B Antibody (011) [NBP2-90260] - Anti-ABHD14B rabbit monoclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Predicted band size:22 kDa. Observed band size:23 kDa.Immunoprecipitation: ABHD14B Antibody (011) [NBP2-90260]
Immunoprecipitation: ABHD14B Antibody (011) [NBP2-90260] - ABHD14B was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. 0.5 uL anti-ABHD14B rabbit monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-ABHD14B rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 25 kDaApplications for ABHD14B Antibody (011)
Application
Recommended Usage
Immunoprecipitation
0.2-1 uL/mg of lysate
Western Blot
1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ABHD14B
Alternate Names
abhydrolase domain containing 14B, abhydrolase domain-containing protein 14B, CCG1-interacting factor B, CIBcell cycle gene 1-interacting factor B, EC 3.-, EC 3.1.11.6, EC 3.1.21.4, MGC15429
Gene Symbol
ABHD14B
Additional ABHD14B Products
Product Documents for ABHD14B Antibody (011)
Product Specific Notices for ABHD14B Antibody (011)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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