ABHD4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06009

Novus Biologicals, part of Bio-Techne
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NBP1-06009
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Key Product Details

Species Reactivity

Human, Mouse, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ABHD4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RPTNPSEIRAP corresponding to internal region according to NP_071343.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ABHD4 Antibody

Immunohistochemistry-Paraffin: ABHD4 Antibody [NBP1-06009]

Immunohistochemistry-Paraffin: ABHD4 Antibody [NBP1-06009]

Immunohistochemistry-Paraffin: ABHD4 Antibody [NBP1-06009] - (3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for ABHD4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:4000
Application Notes
WB: Preliminary experiments gave an approx. 48 kDa band in human brain (cerebellum, cerebral cortex and frontal cortex) lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.8 kDa band according to NP_071343.2. The 48 kDa band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ABHD4

Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis ofN-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains fromN-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate forN-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acylchains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine,lysophosphatidylethanolamine and lysophosphatidylserine

Alternate Names

ABH4, abhydrolase domain containing 4, abhydrolase domain-containing protein 4, Alpha/beta-hydrolase 4, EC 3.1.1.-, FLJ12816, Lyso-N-acylphosphatidylethanolamine lipase

Entrez Gene IDs

63874 (Human); 105501 (Mouse)

Gene Symbol

ABHD4

UniProt

Q8TB40 (Human)

Additional ABHD4 Products

Product Documents for ABHD4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ABHD4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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