ACAT1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51994
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%). Backed by our 100% Guarantee.
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-KRVDFSKVPKLKT corresponding to internal region according to NP_000010.1.
Epitope
C-KRVDFSKVPKLKT
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
45.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ACAT1 Antibody
![Western Blot: ACAT1 Antibody [NBP1-51994]](https://resources.bio-techne.com/images/products/ACAT1-Antibody-Western-Blot-NBP1-51994-img0003.jpg "Western Blot: ACAT1 Antibody [NBP1-51994]")
Western Blot: ACAT1 Antibody [NBP1-51994]
Western Blot: ACAT1 Antibody [NBP1-51994] - Analysis of Mouse (A) and Rat (B) kidney lysates, and Mouse (C) and Rat (D) Liver lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Western Blot: ACAT1 Antibody [NBP1-51994]](https://resources.bio-techne.com/images/products/ACAT1-Antibody-Western-Blot-NBP1-51994-img0002.jpg "Western Blot: ACAT1 Antibody [NBP1-51994]")
Western Blot: ACAT1 Antibody [NBP1-51994]
Western Blot: ACAT1 Antibody [NBP1-51994] - (0.01ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Applications for ACAT1 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:8000
Western Blot
0.01 - 0.03 ug/ml
Application Notes
IF: An anonymous customer has reported particulate cytoplasm staining in HeLa WB: Approx. 45 kDa band observed in human liver lysates and in rodent kidney and liver lysates (calculated MW of 45.2 kDa band according to NP_000010.1).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ACAT1
Long Name
Acetyl-CoA Acetyltransferase 1
Alternate Names
THIL
Entrez Gene IDs
38 (Human)
Gene Symbol
ACAT1
UniProt
Additional ACAT1 Products
Product Documents for ACAT1 Antibody
Product Specific Notices for ACAT1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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