ACAT1 Antibody

Immunocytochemistry/ Immunofluorescence: ACAT1 Antibody [NBP1-89285]

Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-89285] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285]

Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human testis shows strong positivity in mitochondria in cells in seminiferous ducts.

Western Blot: ACAT1 Antibody [NBP1-89285]

Western Blot: ACAT1 Antibody [NBP1-89285] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285]

Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human cerebellum shows strong positivity in mitochondria in purkinje cells.

Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285]

Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human liver shows strong positivity in mitochondria in hepatocytes.

Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285]

Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human kidney shows strong positivity in mitochondria in cells in tubules.

Knockout Validated: ACAT1 Antibody [NBP1-89285]

Knockout Validated: ACAT1 Antibody [NBP1-89285] - ACAT1 protein expression in wild-type and acat-1-/- macrophages. Cell lysates were immunoblotted and ACAT1 protein levels were compared with GAPDH as loading control. CRISPR/Cas-9 used to knockout acat-1. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29390006/) licensed under a CC-BY license.

Western Blot: ACAT1 Antibody [NBP1-89285] -

Western Blot: ACAT1 Antibody [NBP1-89285] - Blocking LD formation increases C. burnetii growth.Wild-type C. burnetii growth in infected MH-S cells treated with different inhibitors was measured at 2 & 4 days post-infection by FFU assay. A-D) Representative images for wild-type MH-S macrophages treated with inhibitors, fixed, stained for PLIN2 (LDs; green) & C. burnetii (red) & imaged day 4 post-treatment at 100X. Scale bar = 10 µm. E) Growth while inhibiting LD formation with triacsin C (10 µM) in wild-type MH-S macrophages. Error bars represent the mean of 4 independent experiments +/- SEM. ** = p <0.01 compared to vehicle-treated cells as determined by two-way ANOVA with Bonferroni post-hoc test. F) ACAT1 protein expression in wild-type & acat-1-/- macrophages. Cell lysates were immunoblotted & ACAT1 protein levels were compared with GAPDH as loading control. G) C. burnetii growth in vehicle-treated wild-type & acat-1-/- MH-S macrophages & (H) T863-treated acat-1-/- MH-S macrophages. Error bars represent the mean of at least 3 independent experiments +/- SEM., * = p<0.05, *** = p <0.001 as determined by two-way ANOVA with Bonferroni post-hoc test. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0192215), licensed under a CC-BY license. Not internally tested by Novus Biologicals.