ACBP Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92856

Novus Biologicals, part of Bio-Techne
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NBP2-92856-0.02ml
NBP2-92856-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ACBP Antibodies »

Product Summary for ACBP Antibody - Azide and BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-104 of human DBI (NP_001171513.1). MWGDLWLLPPASANPGTGTEAEFEKAAEEVRHLKTKPSDEEMLFIYGHYKQATVGDINTERPGMLDFTGKAKWDAWNELKGTSKEDAMKAYINKVEELKKKYGI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ACBP Antibody - Azide and BSA Free

Western Blot: ACBP AntibodyAzide and BSA Free [NBP2-92856]

Western Blot: ACBP AntibodyAzide and BSA Free [NBP2-92856]

Western Blot: ACBP Antibody [NBP2-92856] - Analysis of extracts of various cell lines, using DBI antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.
Immunocytochemistry/ Immunofluorescence: ACBP Antibody - Azide and BSA Free [NBP2-92856]

Immunocytochemistry/ Immunofluorescence: ACBP Antibody - Azide and BSA Free [NBP2-92856]

Immunocytochemistry/Immunofluorescence: ACBP Antibody [NBP2-92856] - Immunofluorescence analysis of U2OS cells using ACBP Rabbit pAb (NBP2-92856) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: ACBP Antibody - Azide and BSA Free [NBP2-92856]

Immunohistochemistry-Paraffin: ACBP Antibody - Azide and BSA Free [NBP2-92856]

Immunohistochemistry-Paraffin: ACBP Antibody [NBP2-92856] - Immunohistochemistry of paraffin-embedded rat ovary using ACBP Rabbit pAb (NBP2-92856) at dilution of 1:250 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Applications for ACBP Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ACBP

ACBP encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. ACBP is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Long Name

Acyl-CoA-binding protein

Alternate Names

DBI, Endozepine, EP

Gene Symbol

DBI

Additional ACBP Products

Product Documents for ACBP Antibody - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for ACBP Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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